Document Detail

Measurement of elastic properties of Xenopus oocytes.
MedLine Citation:
PMID:  9406437     Owner:  NLM     Status:  MEDLINE    
Elastic properties of Xenopus oocytes were examined by measuring intracellular pressure (Pic) and cell volume (Vc) in cells undergoing osmotic swelling. Pic was measured by micropuncture, using the servo-null technique. Vc was obtained by analyzing images acquired from a microscope having a video camera attachment. During osmotic swelling, Pic increased from 61 +/- 17 to 500 +/- 59 Pa (mean +/- SE), but the relationship with volume was not linear. In cells that underwent sequential swelling and shrinking, Pic was always lower on shrinking and the cells showed hysteresis. Cells with vitelline envelope (VE) removed had Pic-Vc curves similar in shape to those of intact cells; however, Pic values were significantly lower. Specific elastance[delta Pic/(delta Vc/Vc)] was reduced by removal of the VE. The data indicate that oocytes are weakly elastic and that a large part of their resistance to expansion resides in the VE.
S M Kelly; Y L Jia; P T Macklem
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Publication Detail:
Type:  In Vitro; Journal Article    
Journal Detail:
Title:  Comparative biochemistry and physiology. Part A, Physiology     Volume:  118     ISSN:  1096-4940     ISO Abbreviation:  Comp. Biochem. Physiol. A Physiol.     Publication Date:  1997 Nov 
Date Detail:
Created Date:  1998-02-18     Completed Date:  1998-02-18     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9516062     Medline TA:  Comp Biochem Physiol A Physiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  607-13     Citation Subset:  IM    
Meakins-Christie Labs, McGill University Clinic, Montreal, Quebec, Canada.
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MeSH Terms
Cell Size
Intracellular Fluid / metabolism
Oocytes / cytology,  metabolism*
Osmotic Pressure
Vitelline Membrane / metabolism
Xenopus laevis

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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