| Measurement of coronary flow reserve in isolated hearts from mice. | |
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MedLine Citation:
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PMID: 15180788 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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AIM: Langendorff-perfused murine hearts are increasingly used in cardiovascular research, but coronary cardiovascular haemodynamics vary considerably from one research group to another. The aim of this study was to establish an isolated, retrogradely perfused mouse heart preparation for the simultaneous measurement of left ventricular haemodynamics and of coronary flow (CF). METHODS: Heart rate was controlled by right atrial pacing (480 beats min(-1)) and heart temperature was kept constant. Accurate flow values of <0.5 mL min(-1) could be determined, and this methodology was then used to study the stability of this preparation, as well as coronary response to vasoactive drugs and to short-term ischaemia. RESULTS: The CF and maximum systolic pressure were well maintained over a 2-h perfusion period, both showing a 10% decline per hour. Sodium-nitroprusside (endothelium-independent) and adenosine (endothelium-dependent) increased CF relatively modest (30-50% above baseline values). Short-term no-flow ischaemia caused a transient 40-50% increase in CF on reperfusion. Peak reflow occurred approximately 15 s after start of reperfusion and flow returned to baseline during the following 1-2 min. Increased coronary blood flow following infusion of vasoactive drugs (nitroprusside or adenosine) or short-term ischaemia were associated with minor changes in ventricular pressure development. CONCLUSIONS: Blood flow and haemodynamics can readily be determined in this isolated perfused mouse heart model, but CF reserve is relatively small, compared with blood-perfused organs. |
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Authors:
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S Bratkovsky; E Aasum; C H Birkeland; R A Riemersma; E S P Myhre; T S Larsen |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Acta physiologica Scandinavica Volume: 181 ISSN: 0001-6772 ISO Abbreviation: Acta Physiol. Scand. Publication Date: 2004 Jun |
Date Detail:
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Created Date: 2004-06-07 Completed Date: 2004-09-20 Revised Date: 2004-11-17 |
Medline Journal Info:
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Nlm Unique ID: 0370362 Medline TA: Acta Physiol Scand Country: England |
Other Details:
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Languages: eng Pagination: 167-72 Citation Subset: IM |
Affiliation:
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Department of Medical Physiology, Faculty of Medicine, University of Tromsø, Norway. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adenosine
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pharmacology Animals Blood Pressure / physiology Coronary Circulation / drug effects, physiology* Mice Myocardial Ischemia / physiopathology Myocardial Reperfusion / methods Nitroprusside / pharmacology Organ Culture Techniques Vasodilator Agents / pharmacology Ventricular Function, Left / physiology* |
| Chemical | |
Reg. No./Substance:
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0/Vasodilator Agents; 15078-28-1/Nitroprusside; 58-61-7/Adenosine |
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