| Measurement of chromogranin A in porcine saliva: validation of a time-resolved immunofluorometric assay and evaluation of its application as a marker of acute stress. | |
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MedLine Citation:
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PMID: 23137814 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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The objective of this study was to develop and validate a time-resolved immunofluorometric assay (TR-IFMA) for porcine salivary chromogranin A (CgA) measurements, using a species-specific antibody, and evaluate its behaviour in an acute stress model. Polyclonal antibodies were produced in rabbits immunized with a synthetic porcine fragment of CgA359-379 and used to develop a sandwich TR-IFMA. This TR-IFMA was analytically validated and showed intra- and inter-assay coefficients of variation of 6.23% and 5.82%, respectively, an analytical limit of detection of 4.27 × 10-3 μg/ml and a limit of quantification of 24.5 × 10-3 μg/ml. The assay also demonstrated a high level of accuracy, as determined by linearity under dilution (r = 0.975) and recovery tests. When a model of experimental acute stress, in which animals were immobilized for 3 min with a nose snare (stressor stimulus), was applied, a significant increase (P < 0.05) in CgA levels in saliva was detected at 15 min post-stressor stimulus. These results indicate that the assay developed in this study could measure CgA in porcine saliva in a reliable way and that the concentrations of CgA in saliva samples of pigs increase after an acute stress situation. |
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Authors:
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D Escribano; L Soler; A M Gutiérrez; S Martínez-Subiela; J J Cerón |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-11-9 |
Journal Detail:
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Title: Animal : an international journal of animal bioscience Volume: - ISSN: 1751-732X ISO Abbreviation: Animal Publication Date: 2012 Nov |
Date Detail:
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Created Date: 2012-11-9 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101303270 Medline TA: Animal Country: - |
Other Details:
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Languages: ENG Pagination: 1-8 Citation Subset: - |
Affiliation:
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1 Department of Animal Medicine and Surgery, Regional Campus of International Excellence 'Campus Mare Nostrum', University of Murcia, 30100 Espinardo, Murcia, Spain. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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