Document Detail

Measurement of the chondrocyte membrane permeability to Me2SO, glycerol and 1,2-propanediol.
MedLine Citation:
PMID:  12835070     Owner:  NLM     Status:  MEDLINE    
The addition of cryopreservative agents (CPAs) to chondrocytes and natural and engineered cartilage is critical to protect the cells and tissues from freezing damage during cryopreservation, but this may cause cell damage, e.g. by osmotic shock. The damage could be minimized by the control of the cell volume excursion with the knowledge of cell membrane permeability. In this study, the cell volume responses of chondrocytes to three commonly used CPAs were evaluated using a perfusion microscope stage. The osmotic response of chondrocytes was measured to the perfusion with 1.4 M dimethyl sulfoxide (Me2SO), 1,2-propanediol and glycerol at 21 degrees C. Cell volumes and their transients were determined with image analysis. The cell membrane permeability parameters, including the hydraulic conductivity (Lp), the CPA permeability (omega) and the reflection coefficients (sigma) in the Kedem-Katchalsky (K-K) model, and the Lp and omega in the two-parameter model were determined. The correlated K-K parameters at 21 degrees C were Lp=0.166 +/- 0.035, 0.149 +/- 0.061, 0.212 +/- 0.041 microm/min atm, omega=(7.630 +/- 0.174) x 10(-2), (1.428 +/- 0.627) x 10(-2), (2.744 +/- 0.775) x 10(-2) microm/s and sigma=0.91 +/- 0.09, 0.82 +/- 0.11, 0.88 +/- 0.10 for Me(2)SO, glycerol and 1,2-propanediol, respectively. For the two-parameter model, the parameter values were Lp=0.163 +/- 0.040, 0.128 +/- 0.031, 0.169 +/- 0.025 microm/min atm, omega=(7.881 +/- 0.178) x 10(-2), (1.529 +/- 0.525) x 10(-2), (3.716 +/- 0.493) x 10(-2) microm/s for Me2SO, glycerol and 1,2-propanediol, respectively. No significant difference in the predictions of cell volume excursion during CPA addition was observed when using either the K-K model or the two-parameter model and it was hence advised to adopt the simple two-parameter model in the evaluation. The measured parameters can be used to optimise the CPA addition and removal protocols to maximize the cell survival during cryopreservation.
Xia Xu; Zhanfeng Cui; Jill P G Urban
Publication Detail:
Type:  Comparative Study; Evaluation Studies; In Vitro; Journal Article; Research Support, Non-U.S. Gov't; Validation Studies    
Journal Detail:
Title:  Medical engineering & physics     Volume:  25     ISSN:  1350-4533     ISO Abbreviation:  Med Eng Phys     Publication Date:  2003 Sep 
Date Detail:
Created Date:  2003-07-01     Completed Date:  2004-04-06     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  9422753     Medline TA:  Med Eng Phys     Country:  England    
Other Details:
Languages:  eng     Pagination:  573-9     Citation Subset:  IM    
Department of Engineering Science, Oxford University, Parks Road, OX1 3PJ, Oxford, UK.
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MeSH Terms
Cell Membrane Permeability / drug effects*,  physiology*
Cell Size / drug effects
Cell Survival / drug effects
Cells, Cultured
Chondrocytes / cytology,  drug effects*,  metabolism
Cold Temperature
Computer Simulation
Cryopreservation / methods*
Cryoprotective Agents / pharmacokinetics,  pharmacology
Dimethyl Sulfoxide / pharmacokinetics,  pharmacology*
Glycerol / pharmacokinetics,  pharmacology*
Models, Biological*
Propylene Glycol / pharmacokinetics,  pharmacology*
Reg. No./Substance:
0/Cryoprotective Agents; 56-81-5/Glycerol; 57-55-6/Propylene Glycol; 67-68-5/Dimethyl Sulfoxide

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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