| Measurement of chemical analytes in vitreous humor: stability and precision studies. | |
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MedLine Citation:
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PMID: 15027563 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The analytic accuracy and precision for measurement of chemical analytes in vitreous humor (VH) are critical if results are to be used in forensic pathology. The purpose of our study was to demonstrate the stability and the reproducibility of VH sodium, potassium, chloride, glucose, urea nitrogen, acetone, and beta-hydroxybutyrate in specimens obtained from both eyes in medical examiner cases. We also compared with calculated VH osmolalities. Small but significant increases were observed in VH electrolyte concentrations in specimens refrigerated 6-12 months: sodium pre 144 mmol/L, post 151 mmol/L; potassium pre 12.0 mmol/L, post 12.8 mmol/L; chloride pre 121 mmol/L, post 123 mmol/L. No differences were observed between eyes, and within-day precision for all electrolyte measurements were excellent, (<1%). Frozen specimens showed significantly higher measured (439 mOsmol/kg) as compared with calculated osmolality (305 mOsmol/kg), with 1% within-day precision and no significance between eye variation for glucose and urea nitrogen. In 20 of 24 medical cases selected for possible ketoacidosis, measurement of beta-hydroxybutyrate concentrations appears to be a promising diagnostic biomarker for confirming suspected ketosis in medical examiner cases by means of VH analysis. |
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Authors:
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Angelique Gagajewski; MaryAnn M Murakami; Julie Kloss; Michael Edstrom; Melissa Hillyer; Garry F Peterson; Janis Amatuzio; Fred S Apple |
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Publication Detail:
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Type: Evaluation Studies; Journal Article |
Journal Detail:
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Title: Journal of forensic sciences Volume: 49 ISSN: 0022-1198 ISO Abbreviation: J. Forensic Sci. Publication Date: 2004 Mar |
Date Detail:
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Created Date: 2004-03-18 Completed Date: 2004-04-22 Revised Date: 2004-11-17 |
Medline Journal Info:
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Nlm Unique ID: 0375370 Medline TA: J Forensic Sci Country: United States |
Other Details:
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Languages: eng Pagination: 371-4 Citation Subset: IM |
Affiliation:
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University of Minnesota School of Medicine, Minneapolis, MN, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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3-Hydroxybutyric Acid
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analysis Acetone / analysis Chlorides / analysis Forensic Medicine Glucose / analysis Humans Nitrogen / analysis Osmolar Concentration Postmortem Changes Potassium / analysis Refrigeration Reproducibility of Results Sodium / analysis Urea / analysis Vitreous Body / chemistry* Water-Electrolyte Balance |
| Chemical | |
Reg. No./Substance:
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0/Chlorides; 300-85-6/3-Hydroxybutyric Acid; 50-99-7/Glucose; 57-13-6/Urea; 67-64-1/Acetone; 7440-09-7/Potassium; 7440-23-5/Sodium; 7727-37-9/Nitrogen |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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