| Measurement of cerebral blood volume in mouse brain regions using micro-computed tomography. | |
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MedLine Citation:
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PMID: 19362597 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Micro-computed tomography (micro-CT) is an X-ray imaging technique that can produce detailed 3D images of cerebral vasculature. This paper describes the development of a novel method for using micro-CT to measure cerebral blood volume (CBV) in the mouse brain. As an application of the methodology, we test the hypotheses that differences in CBV exist over anatomical brain regions and that high energy demanding primary sensory regions of the cortex have locally elevated CBV, which may reflect a vascular specialization. CBV was measured as the percentage of tissue space occupied by a radio-opaque silicon rubber that fills the vasculature. To ensure accuracy of the CBV measurements, several innovative refinements were made to standard micro-CT specimen preparation and analysis procedures. Key features of the described method are vascular perfusion under controlled pressure, registration of the micro-CT images to an MRI anatomical brain atlas and re-scaling of micro-CT intensities to CBV units with selectable exclusion of major vessels. Histological validation of the vascular perfusion showed that the average percentage of vessels filled was 93+/-3%. Comparison of thirteen brain regions in nine mice revealed significant differences in CBV between regions (p<0.0001) while cortical maps showed that primary visual and auditory areas have higher CBV than primary somatosensory areas. |
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Authors:
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Brige P Chugh; Jason P Lerch; Lisa X Yu; Martin Pienkowski; Robert V Harrison; R Mark Henkelman; John G Sled |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-04-09 |
Journal Detail:
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Title: NeuroImage Volume: 47 ISSN: 1095-9572 ISO Abbreviation: Neuroimage Publication Date: 2009 Oct |
Date Detail:
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Created Date: 2009-07-31 Completed Date: 2009-10-15 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9215515 Medline TA: Neuroimage Country: United States |
Other Details:
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Languages: eng Pagination: 1312-8 Citation Subset: IM |
Affiliation:
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Mouse Imaging Centre, The Hospital for Sick Children, Department of Medical Biophysics, University of Toronto, Toronto, Ontario, Canada. brige@phenogenomics.ca |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Blood Volume / physiology* Blood Volume Determination / methods* Brain / blood supply, physiology* Cerebrovascular Circulation / physiology Female Imaging, Three-Dimensional / methods* Mice Mice, Inbred C57BL Perfusion Imaging / methods Radiographic Image Interpretation, Computer-Assisted / methods Tomography, X-Ray Computed / methods*, veterinary* |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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