| Measurement of cell volume loss in the liquid region preceding an advancing phase change interface. | |
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MedLine Citation:
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PMID: 9988671 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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It is well understood that the solidification of a solution results in a redistribution of solute in the liquid zone. For the freezing of suspensions of cells it is anticipated that accumulation of solute in the region leading a growing ice phase will cause an osmotic response in cells before the ice phase reaches the cells. To measure this phenomenon in a specific algal species, the volume changes in Chlorococcum texanum during freezing were studied using directional solidification cryomicroscopy. The relative cell volume was tracked continuously as a function of temperature and position as cells encountered the moving phase front. The loss of cell volume was measured in the liquid region containing concentrated solute ahead of the growing solid phase. |
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Authors:
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H R Harmison; K R Diller; J R Walsh; C M Neils; J J Brand |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. |
Journal Detail:
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Title: Annals of the New York Academy of Sciences Volume: 858 ISSN: 0077-8923 ISO Abbreviation: Ann. N. Y. Acad. Sci. Publication Date: 1998 Sep |
Date Detail:
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Created Date: 1999-02-11 Completed Date: 1999-02-11 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 7506858 Medline TA: Ann N Y Acad Sci Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 276-83 Citation Subset: IM |
Affiliation:
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Biomedical Engineering Program, University of Texas, Austin 78712, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Algae, Green
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cytology*,
physiology* Cell Size* Cryopreservation / methods* Freezing Microscopy / methods |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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