Document Detail


Measurement of cell growth by a rapid U.V. spectrophotometric method.
MedLine Citation:
PMID:  1000027     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A spectrophotometric method for measuring mammalian cell growth in culture is described. The attached cells were solubilized in 2N sodium hydroxide and the absorption was measured at 225 nm. The technique is sensitive to about 3000 cells per Petri dish and a linear relationship was obtained between the number of cells and the absorption at this wave length. The method is rapid and the solubilized material can be recovered for liquid scintillation counting when needed.
Authors:
M Pagé; C Dufour
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Biomedicine / [publiée pour l'A.A.I.C.I.G.]     Volume:  25     ISSN:  0300-0893     ISO Abbreviation:  Biomedicine     Publication Date:  1976 Jul 
Date Detail:
Created Date:  1977-02-26     Completed Date:  1977-02-26     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  0361342     Medline TA:  Biomedicine     Country:  FRANCE    
Other Details:
Languages:  eng     Pagination:  170-3     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Count / methods*
Cell Division
Cell Line
Cells, Cultured
Hela Cells
Humans
Liver / cytology
Rats
Spectrophotometry, Ultraviolet

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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