| Measurement of cell growth by a rapid U.V. spectrophotometric method. | |
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MedLine Citation:
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PMID: 1000027 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A spectrophotometric method for measuring mammalian cell growth in culture is described. The attached cells were solubilized in 2N sodium hydroxide and the absorption was measured at 225 nm. The technique is sensitive to about 3000 cells per Petri dish and a linear relationship was obtained between the number of cells and the absorption at this wave length. The method is rapid and the solubilized material can be recovered for liquid scintillation counting when needed. |
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Authors:
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M Pagé; C Dufour |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Biomedicine / [publiée pour l'A.A.I.C.I.G.] Volume: 25 ISSN: 0300-0893 ISO Abbreviation: Biomedicine Publication Date: 1976 Jul |
Date Detail:
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Created Date: 1977-02-26 Completed Date: 1977-02-26 Revised Date: 2004-11-17 |
Medline Journal Info:
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Nlm Unique ID: 0361342 Medline TA: Biomedicine Country: FRANCE |
Other Details:
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Languages: eng Pagination: 170-3 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Count / methods* Cell Division Cell Line Cells, Cultured Hela Cells Humans Liver / cytology Rats Spectrophotometry, Ultraviolet |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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