Document Detail


Measurement of cell death in Mammalian cells.
MedLine Citation:
PMID:  22382997     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Methods for assessing mammalian cell death are presented in this unit. The unit is divided into six sections: (1) a brief overview of cytotoxicity and pathways of cell death, (2) a method to measure cell death using lactate dehydrogenase (LDH) release as a marker of membrane integrity, (3) a flow cytometry method that simultaneously measures two types of cell death, necrosis, and apoptosis, (4) use of fluorescence microscopy and nuclear morphology to assess apoptosis and necrosis, (5) the use of multi-well plates and high-content analysis imaging systems to assess nuclear morphology, and (6) a discussion of the use of cytotoxicity assays to determine the mechanisms of cell death. Curr. Protoc. Pharmacol. 56:12.8.1-12.8.24. © 2012 by John Wiley & Sons, Inc.
Authors:
Brian S Cummings; Lauren P Wills; Rick G Schnellmann
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Current protocols in pharmacology / editorial board, S.J. Enna (editor-in-chief) ... [et al.]     Volume:  Chapter 12     ISSN:  1934-8290     ISO Abbreviation:  Curr Protoc Pharmacol     Publication Date:  2012 Mar 
Date Detail:
Created Date:  2012-03-02     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9717249     Medline TA:  Curr Protoc Pharmacol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  Unit12.8     Citation Subset:  IM    
Affiliation:
Department of Pharmaceutical and Biomedical Sciences, University of Georgia, Athens, Georgia.
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