Document Detail


Measurement of the cariogenicity of snacks using a radioisotope PAHA disc.
MedLine Citation:
PMID:  22177337     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
OBJECTIVES: This study aimed to establish a method for measuring the cariogenic potential of foods with high reproducibility in vitro. METHODS: Streptococcus mutans (S. mutans) was incubated in test foods with radioisotope polyacrylamide hydroxyapatite (PAHA) for 150min at 37°C. Then, the amount of radioisotope (32)P released from PAHA was measured using a liquid scintillation counter and scanning electron microscopy (SEM). RESULTS: The radioisotope PAHA discs that were soaked in 10% sucrose solutions had a high cariogenic potential and showed a remarkably demineralized surface (p<0.05). The radioisotope PAHA disc that was incubated with snacks that had a high cariogenic potential showed a remarkably demineralized surface via SEM. Candy had a relatively high cariogenic potential, whereas xylitol gum had a relatively low potential. CONCLUSIONS: The cariogenicity of snacks can easily be evaluated by measuring the amount of (32)P released from radioisotope PAHA discs.
Authors:
Eun-Jung Lee; Hye-Kyoung Yun; In-Kyeong Hwang; Kwang-Hak Bae; Bo-Hyoung Jin; Dai-Il Paik
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-12-15
Journal Detail:
Title:  Archives of oral biology     Volume:  -     ISSN:  1879-1506     ISO Abbreviation:  -     Publication Date:  2011 Dec 
Date Detail:
Created Date:  2011-12-19     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0116711     Medline TA:  Arch Oral Biol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2011. Published by Elsevier Ltd.
Affiliation:
Department of Preventive & Social Dentistry, School of Dentistry, Seoul National University,South Korea; Department of Food & Nutrition, College of Human Ecology, Seoul National University,South Korea.
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