| Measurement of caffeic and ferulic acid equivalents in plasma after coffee consumption: small intestine and colon are key sites for coffee metabolism. | |
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MedLine Citation:
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PMID: 19937852 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Previous studies on coffee examined absorption of phenolic acids (PA) in the small intestine, but not the contribution of the colon to absorption. Nine healthy volunteers ingested instant soluble coffee ( approximately 335 mg total chlorogenic acids (CGAs)) in water. Blood samples were taken over 12 h, and at 24 h to assess return to baseline. Many previous studies, which used glucuronidase and sulfatase, measured only PA and did not rigorously assess CGAs. To improve this, plasma samples were analyzed after full hydrolysis by chlorogenate esterase, glucuronidase and sulfatase to release aglycone equivalents of PA followed by liquid-liquid extraction and ESI-LC-ESI-MS/MS detection. Ferulic, caffeic and isoferulic acid equivalents appeared rapidly in plasma, peaking at 1-2 h. Dihydrocaffeic and dihydroferulic acids appeared in plasma 6-8 h after ingestion (T(max=)8-12 h). Substantial variability in maximum plasma concentration and T(max) was also observed between individuals. This study confirms that the small intestine is a significant site for absorption of PA, but shows for the first time that the colon/microflora play the major role in absorption and metabolism of CGAs and PA from coffee. |
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Authors:
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Mathieu Renouf; Philippe A Guy; Cynthia Marmet; Anne-Lise Fraering; Karin Longet; Julie Moulin; Marc Enslen; Denis Barron; Fabiola Dionisi; Christophe Cavin; Gary Williamson; Heike Steiling |
Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Molecular nutrition & food research Volume: 54 ISSN: 1613-4133 ISO Abbreviation: Mol Nutr Food Res Publication Date: 2010 Jun |
Date Detail:
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Created Date: 2010-06-14 Completed Date: 2010-09-16 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101231818 Medline TA: Mol Nutr Food Res Country: Germany |
Other Details:
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Languages: eng Pagination: 760-6 Citation Subset: IM |
Affiliation:
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Nestlé Research Center, Nestec Limited, Vers-Chez-Les-Blanc, Lausanne, Switzerland. mathieu.renouf@rdls.nestle.com |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adult Caffeic Acids / blood* Chlorogenic Acid / blood Chromatography, High Pressure Liquid Coffee / metabolism* Colon / metabolism* Coumaric Acids / blood* Female Humans Intestine, Small / metabolism* Male Spectrometry, Mass, Electrospray Ionization |
| Chemical | |
Reg. No./Substance:
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0/Caffeic Acids; 0/Coffee; 0/Coumaric Acids; 1135-24-6/ferulic acid; 327-97-9/Chlorogenic Acid; 331-39-5/caffeic acid |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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