Document Detail


Measurement of c-myc protein content and cell cycle kinetics of normal and spontaneously transformed murine mastocytes by bivariate flow cytometry.
MedLine Citation:
PMID:  2245444     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Progressive in vitro culturing of interleukin-3 (IL-3) dependent normal murine mastocytes (PB-3) resulted in a variant cell line (PB-1) able to grow without exogenous IL-3 and which was tumorogenic in syngenic mice. Bivariate flow cytometry was used to evaluate the c-myc protein and DNA content of PB-3 and PB-1 cells. The c-myc protein was detected by specific monoclonal antibodies. Kinetic characteristics of PB-3 and PB-1 cell lines, namely, the duration of the G1, S and G2 + M cell cycle phases were also evaluated using the bromodeoxyuridine (BrdU) pulse-chase method and BrdU/DNA flow cytometry. Levels of c-myc protein in PB-1 cells were about two-fold higher than those of PB-3 cells in all cell cycle phases. Mean duration of the cell cycle (Tc) was 15.3 h for PB-3 cells and 12.4 h for PB-1 cells. Shortening in Tc for the transformed cells was due to a decrease of nearly 30% in mean duration of the G1 phase (from 8 h to 5.7 h). No significant differences were found in the duration of the S and G2 + M phases. These results indicate that acquired IL-3 independency in vitro and tumorogenicity of PB-1 cells were accompanied by a doubling of c-myc protein level and by a parallel shortening, or bypass, of the regulatory events within the G1 phase of the cell cycle.
Authors:
W Giaretti; A Di Vinci; E Geido; B Marsano; M Minks; S Bruno
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cell and tissue kinetics     Volume:  23     ISSN:  0008-8730     ISO Abbreviation:  Cell Tissue Kinet     Publication Date:  1990 Sep 
Date Detail:
Created Date:  1991-01-10     Completed Date:  1991-01-10     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0174107     Medline TA:  Cell Tissue Kinet     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  473-85     Citation Subset:  IM    
Affiliation:
Biophysics Laboratory, National Institute for Cancer Research (I.S.T.), Genoa, Italy.
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MeSH Terms
Descriptor/Qualifier:
Animals
Antibodies, Monoclonal / diagnostic use
Bromodeoxyuridine / diagnostic use,  metabolism
Cell Cycle / drug effects
Cell Line, Transformed
Cell Nucleus / chemistry
DNA / analysis,  metabolism
Female
Flow Cytometry / methods
Kinetics
Mast Cells / chemistry*,  metabolism,  physiology
Mice
Mice, Inbred DBA
Propidium / diagnostic use
Proto-Oncogene Proteins c-myc / analysis*,  metabolism
Chemical
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Proto-Oncogene Proteins c-myc; 36015-30-2/Propidium; 59-14-3/Bromodeoxyuridine; 9007-49-2/DNA

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