| Measurement of c-myc protein content and cell cycle kinetics of normal and spontaneously transformed murine mastocytes by bivariate flow cytometry. | |
MedLine Citation:
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PMID: 2245444 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Progressive in vitro culturing of interleukin-3 (IL-3) dependent normal murine mastocytes (PB-3) resulted in a variant cell line (PB-1) able to grow without exogenous IL-3 and which was tumorogenic in syngenic mice. Bivariate flow cytometry was used to evaluate the c-myc protein and DNA content of PB-3 and PB-1 cells. The c-myc protein was detected by specific monoclonal antibodies. Kinetic characteristics of PB-3 and PB-1 cell lines, namely, the duration of the G1, S and G2 + M cell cycle phases were also evaluated using the bromodeoxyuridine (BrdU) pulse-chase method and BrdU/DNA flow cytometry. Levels of c-myc protein in PB-1 cells were about two-fold higher than those of PB-3 cells in all cell cycle phases. Mean duration of the cell cycle (Tc) was 15.3 h for PB-3 cells and 12.4 h for PB-1 cells. Shortening in Tc for the transformed cells was due to a decrease of nearly 30% in mean duration of the G1 phase (from 8 h to 5.7 h). No significant differences were found in the duration of the S and G2 + M phases. These results indicate that acquired IL-3 independency in vitro and tumorogenicity of PB-1 cells were accompanied by a doubling of c-myc protein level and by a parallel shortening, or bypass, of the regulatory events within the G1 phase of the cell cycle. |
Authors:
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W Giaretti; A Di Vinci; E Geido; B Marsano; M Minks; S Bruno |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Cell and tissue kinetics Volume: 23 ISSN: 0008-8730 ISO Abbreviation: Cell Tissue Kinet Publication Date: 1990 Sep |
Date Detail:
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Created Date: 1991-01-10 Completed Date: 1991-01-10 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0174107 Medline TA: Cell Tissue Kinet Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 473-85 Citation Subset: IM |
Affiliation:
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Biophysics Laboratory, National Institute for Cancer Research (I.S.T.), Genoa, Italy. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antibodies, Monoclonal / diagnostic use Bromodeoxyuridine / diagnostic use, metabolism Cell Cycle / drug effects Cell Line, Transformed Cell Nucleus / chemistry DNA / analysis, metabolism Female Flow Cytometry / methods Kinetics Mast Cells / chemistry*, metabolism, physiology Mice Mice, Inbred DBA Propidium / diagnostic use Proto-Oncogene Proteins c-myc / analysis*, metabolism |
| Chemical | |
Reg. No./Substance:
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0/Antibodies, Monoclonal; 0/Proto-Oncogene Proteins c-myc; 36015-30-2/Propidium; 59-14-3/Bromodeoxyuridine; 9007-49-2/DNA |
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