| Measurement by ELISA of complement factor 4 (C4) in the rat brain: necessity for removal of cerebrovascular proteins. | |
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MedLine Citation:
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PMID: 16847591 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Assessment of complement 4 (C4) levels in experimental animals is used as a marker for activation of the classical complement pathway. The objective of this study was to develop a method for measuring C4 concentrations in the rat brain. An ELISA (sensitivity = 0.5 ng C4/ml) was used to measure C4 in regional brain homogenates from Fischer rats cardiac-perfused with phosphate buffered saline to remove cerebrovascular contents, and from sham-perfused rats. Ventral midbrain C4 levels were increased (p < 0.001) versus frontal cortex and striatum in sham-perfused rats, whereas after perfusion there were no differences between brain regions. Removal of cerebrovascular contents decreased C4 by 43% in striatum, 52% in frontal cortex, and 69% in ventral midbrain (all p < 0.01 versus sham-perfused means). These results indicate that C4 in the rat brain can be measured quantitatively by ELISA provided that cerebrovascular proteins are removed by perfusion. |
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Authors:
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David A Loeffler; Dianne M Camp |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2006-07-18 |
Journal Detail:
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Title: Neurochemical research Volume: 31 ISSN: 0364-3190 ISO Abbreviation: Neurochem. Res. Publication Date: 2006 Jul |
Date Detail:
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Created Date: 2006-07-27 Completed Date: 2006-10-03 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 7613461 Medline TA: Neurochem Res Country: United States |
Other Details:
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Languages: eng Pagination: 999-1002 Citation Subset: IM |
Affiliation:
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Division of Neurology, William Beaumont Hospital Research Institute, 3811 West Thirteen Mile Rd., Royal Oak, MI 48073, USA. DLoeffler@beaumont.edu |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Brain / metabolism* Complement C4 / metabolism* Enzyme-Linked Immunosorbent Assay Male Nerve Tissue Proteins / isolation & purification* Rats Rats, Inbred F344 |
| Chemical | |
Reg. No./Substance:
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0/Complement C4; 0/Nerve Tissue Proteins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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