| Measurement of blood acetoacetate and beta-hydroxybutyrate in an automatic analyser. | |
| | |
| Jump to Full Text | |
MedLine Citation:
|
PMID: 18924878 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
|
g-hydroxybutyrate and acetoacetate as well as lactate and pyruvate are intermediary metabolites normally present in blood. The g-hydroxybutyrate/acetoacetate ratio is an expression of the mitochondrial oxido-reduction state. This ketone body ratio can provide a clue to diagnosis and metabolic status in congenital errors of the electron transport chain and pyruvate metabolism. The standardization of these analytical procedures improves the interpretation of the results helping in the difficult diagnosis of mitochondrial diseases in children. This study describes an adaptation to a Dimension R 2 L (Dade Behring, Newark, Delaware, USA) automatic analyser for a method to measure blood ketone bodies (g-hydroxybutyrate and acetoacetate). The method allows the metabolites to be measured directly in nondeproteinized plasma (fluoride/ethylenediaminetetraacetic acid). This adaptation simplifies the analytical procedure and limits the turnaround time to 20 minutes. With a sample volume of 200 mu l metabolite concentrations ranging from 12 to 1300 mu molL(-1) of g-hydroxybutyrate and from 10 to 450 mu molL(-1) of acetoacetate may be measured with a reliable analytical response. |
| | |
Authors:
|
A Galán; J Hernández; O Jimenez |
Related Documents
:
|
14979418 - Ph-stat ventilation management: a simple method of achieving this regimen. 15558748 - Ex vivo nmr study of lactate metabolism in rat brain under various depressed states. 1778908 - Intra- and extracellular ph of the brain in vivo studied by 31p-nmr during hyper- and h... 6773028 - Facilitated diffusion of lactic acid in the guinea-pig placenta. 6515128 - Effect of oxygen-carrying resuscitation fluids on the pharmacokinetics of antipyrine, d... 1285368 - The effect of somatostatin, gabexate mesilate and dextran 40 on the microcirculation in... |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: Journal of automated methods & management in chemistry Volume: 23 ISSN: 1463-9246 ISO Abbreviation: J Autom Methods Manag Chem Publication Date: 2001 |
Date Detail:
|
Created Date: 2008-10-17 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 100885454 Medline TA: J Autom Methods Manag Chem Country: United States |
Other Details:
|
Languages: eng Pagination: 69-76 Citation Subset: - |
Affiliation:
|
Clinical Biochemistry Service Hospital Universitario Germans Trias i Pujol Carretera del Canyet s/n Badalona Barcelona Spain. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
| Full Text | |
|
Journal Information Journal ID (nlm-ta): J Autom Methods Manag Chem ISSN: 1463-9246 ISSN: 1464-5068 Publisher: Hindawi Publishing Corporation |
Article Information Download PDF  Copyright ? 2001 Hindawi Publishing Corporation. open-access: This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Print publication date: Year: 2001 Volume: 23 Issue: 3 First Page: 69 Last Page: 76 ID: 2548350 PubMed Id: 18924878 Publisher Item Identifier: S1463924601000086 DOI: 10.1155/S1463924601000086 |
| Measurement of blood acetoacetate and ?-hydroxybutyrate in an automatic analyser | |
| Amparo Gal?n |
Address: agalan@ns.hugtip.scs.es |
| Jos?m. Hern?ndez | |
| Orlando Jimenez | |
|
Clinical Biochemistry ServiceHospital Universitario Germans Trias i PujolCarretera del Canyet s/nBadalonaBarcelonaSpain |
|
Article Categories:
|
|
Previous Document: Hirudin as an anticoagulant for both haematology and chemistry tests.
Next Document: Evaluation of use of the optional unit QA-810V for the determination of five-part leukocyte differen...