Document Detail

Measurement and autocorrelation analysis of two-dimensional light-scattering patterns from living cells for label-free classification.
MedLine Citation:
PMID:  21387543     Owner:  NLM     Status:  Publisher    
We incorporate optics and an ICCD to record the two-dimensional angular optical scattering (TAOS) patterns retrieved from single aerosolized cells. We analyze these patterns by performing autocorrelations and demonstrate that we are able to retrieve cell size from the locations of the secondary maxima. Additional morphological information is contained in the autocorrelation functions and decay rate of the heights of the autocorrelation peaks. We demonstrate these techniques with C6 and Y79 cells, which are readily distinguishable. One key advantage of this methodology is that there is no requirement for antibody and fluorescent labeling molecules. Published 2011 Wiley-Liss, Inc.
Yong-Le Pan; Matthew J Berg; Samuel Shao-Min Zhang; Heeso Noh; Hui Cao; Richard K Chang; Gorden Videen
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-3-8
Journal Detail:
Title:  Cytometry. Part A : the journal of the International Society for Analytical Cytology     Volume:  -     ISSN:  1552-4930     ISO Abbreviation:  -     Publication Date:  2011 Mar 
Date Detail:
Created Date:  2011-3-9     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101235694     Medline TA:  Cytometry A     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Published 2011 Wiley-Liss, Inc.
U. S. Army Research Laboratory, Adelphi, Maryland 20783.
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