Document Detail


Measurement of ascorbic acid in human plasma and serum: stability, intralaboratory repeatability, and interlaboratory reproducibility.
MedLine Citation:
PMID:  8697586     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We demonstrate that total ascorbic acid (TAA, the sum of ascorbic acid and dehydroascorbic acid) in properly prepared human plasma is stable at -70 degrees C for at least 6 years when preserved with dithiothreitol. TAA in human plasma or serum preserved with metaphosphoric acid degrades slowly, at the rate of no more than 1% per year. As assessed from our stability data and from data obtained from 23 laboratories over a period of > 2 years, the intralaboratory repeatability of TAA measurement is approximately 2 mumol/L, irrespective of TAA concentration. Nonchromatographic analytical methods involving dinitrophenylhydrazine and 0-phenylenediamine yield biased results relative to chromatographic methods. Within groups of laboratories that use roughly similar analytical methods, the interlaboratory measurement reproducibility CV for TAA is 15%.
Authors:
S A Margolis; D L Duewer
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Clinical chemistry     Volume:  42     ISSN:  0009-9147     ISO Abbreviation:  Clin. Chem.     Publication Date:  1996 Aug 
Date Detail:
Created Date:  1996-09-05     Completed Date:  1996-09-05     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  9421549     Medline TA:  Clin Chem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1257-62     Citation Subset:  IM    
Affiliation:
Analytical Chemistry Division, Chemical Science and Technology Laboratory, National Institute of Standards and Technology, Gaithersburg, MD 20899, USA. Sam.Margolis@NIST.gov
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MeSH Terms
Descriptor/Qualifier:
Ascorbic Acid / blood*
Chromatography / methods,  statistics & numerical data
Dehydroascorbic Acid / blood
Dithiothreitol
Drug Stability
Freezing
Humans
Kinetics
Laboratories / standards*
Phenylenediamines
Phenylhydrazines
Phosphorous Acids
Plasma
Reproducibility of Results
Specimen Handling
Stereoisomerism
Time Factors
Grant Support
ID/Acronym/Agency:
CP9-0506/CP/NCI NIH HHS
Chemical
Reg. No./Substance:
0/Phenylenediamines; 0/Phenylhydrazines; 0/Phosphorous Acids; 10343-62-1/metaphosphoric acid; 119-26-6/2,4-dinitrophenylhydrazine; 3483-12-3/Dithiothreitol; 490-83-5/Dehydroascorbic Acid; 50-81-7/Ascorbic Acid; 95-54-5/1,2-diaminobenzene

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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