| Measurement of apolipoprotein B synthesis in perfused rat liver using stable isotopes: [15N]hippurate as a measure of the intracellular [15N]glycine precursor enrichment. | |
| | |
MedLine Citation:
|
PMID: 2794777 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Rat livers were perfused by the nonrecirculating technique with medium containing [15N]glycine and sodium benzoate. At various times, the isotopic enrichment of hepatic free glycine, hepatic glycyl-tRNA, and perfusate hippurate was measured by GLC-MS. After 60 min, these parameters had reached approximately maximal values. At 90 min, the perfusate hippurate had a 30% greater enrichment of 15N than the intracellular glycine or glycyl-tRNA. Hippurate enrichment was half that of the medium glycine. The rat livers secreted apolipoprotein B (B-100 plus B-48) at a rate of 22 micrograms/g per h. From the 15N enrichment and the secretion rate, an intrahepatic pool size of 86 micrograms/g of apoB was calculated. From the minimal intracellular transit time of 30 min, an apoB fractional synthetic rate (FSR) of 2 pools/h was indicated, whereas the FSR estimated from the 15N-enrichment was 0.26/h. A possible explanation for the discrepancy is that apoB may recycle within the hepatocyte. On the basis of the present experiments, when hippurate enrichment is used as a measure of the enrichment of intrahepatic glycine in in vivo studies with 15N-labeled glycine, a correction should be applied, under normal metabolic circumstances, of approximately 20-30%. |
| | |
Authors:
|
T Matsushima; D R Cryer; K E Winkler; J B Marsh; J A Cortner |
Related Documents
:
|
3093357 - Effect of 16,16-dimethyl prostaglandin e2 on oxygen uptake and microcirculation in the ... 21601937 - Cxcl12 expression within the cns contributes to the resistance against experimental aut... 1220697 - Acute effects of carbon monoxide on the metabolism of perfused rat liver. 6109737 - Clearance of immunoreactive somatostatin by perfused rat liver. 11345957 - Contribution of the cholinergic basal forebrain to proactive interference from stored o... 8095537 - Antidiarrhoeic activity of quercitrin in mice and rats. |
Publication Detail:
|
Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
|
Title: Journal of lipid research Volume: 30 ISSN: 0022-2275 ISO Abbreviation: J. Lipid Res. Publication Date: 1989 Jun |
Date Detail:
|
Created Date: 1989-11-03 Completed Date: 1989-11-03 Revised Date: 2007-11-14 |
Medline Journal Info:
|
Nlm Unique ID: 0376606 Medline TA: J Lipid Res Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 841-6 Citation Subset: IM |
Affiliation:
|
Department of Physiology and Biochemistry, Medical College of Pennsylvania, Philadelphia 19129. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Apolipoproteins B / biosynthesis* Chromatography, Ion Exchange Gas Chromatography-Mass Spectrometry Glycine / metabolism* Hippurates / analysis* Liver / metabolism* Male Nitrogen Isotopes Perfusion RNA, Transfer, Gly / analysis Rats Rats, Inbred Strains Time Factors |
| Grant Support | |
ID/Acronym/Agency:
|
HL 22633/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/Apolipoproteins B; 0/Hippurates; 0/Nitrogen Isotopes; 0/RNA, Transfer, Gly; 56-40-6/Glycine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Binding of apoE-rich high density lipoprotein particles by saturable sites on human blood platelets ...
Next Document: Identification of 3 alpha,4 beta,7 alpha-trihydroxy-5 beta-cholanoic acid in human bile: reflection ...