Document Detail


Measurement of amniotic fluid surfactant.
MedLine Citation:
PMID:  6269778     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Measurement of the production of surfactant is the most direct means of prenatally assessing fetal pulmonary maturity. We review assays which have evolved for measuring surfactant, classifying them into two general categories: biochemical quantitation and biophysical measurements. Biochemical quantitation assays include the amniotic fluid lecithin/sphingomyelin ratio and quantitation of lecithin and other surfactant phospholipids. Biophysical measurements include measurement of surface-tension-lowering ability of extracts of amniotic fluid lipid and evaluation of surface-tension-related properties such as foam stability and microviscosity. Assays of surfactant are subject to certain pre-analysis sources of variation over which the analyst has no control, such as variability in total in vivo amniotic fluid volume, incomplete in vivo mixing of surfactant with amniotic fluid, and presence of contaminating blood or meconium. We also examine other factors such as centrifugation speed and time, and storage of the amniotic specimen before analysis. These factors can dramatically affect analyses, and must be carefully controlled by the analyst. In general, both biochemical and biophysical approaches to surfactant analysis are useful diagnostically. When properly performed, both approaches give results that correlate well, both with each other and with clinical outcome. Because "mature" and "immature" values overlap, none of the assays can completely eliminate false predictions, whether of fetal maturity or fetal immaturity.
Authors:
D E Freer; B E Statland
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Publication Detail:
Type:  Comparative Study; Journal Article; Review    
Journal Detail:
Title:  Clinical chemistry     Volume:  27     ISSN:  0009-9147     ISO Abbreviation:  Clin. Chem.     Publication Date:  1981 Oct 
Date Detail:
Created Date:  1981-12-15     Completed Date:  1981-12-15     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9421549     Medline TA:  Clin Chem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1629-41     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Amniotic Fluid / analysis*
Blood
Centrifugation / methods
False Positive Reactions
Fatty Acids / analysis
Female
Fetal Organ Maturity
Fluorescence Polarization
Gestational Age
Humans
Hyaline Membrane Disease / diagnosis
Inclusion Bodies / analysis
Infant, Newborn
Lung / embryology*
Meconium
Phosphatidylcholines / analysis
Phosphatidylglycerols / analysis
Pregnancy
Prenatal Diagnosis / methods*
Pulmonary Surfactants / analysis*
Reference Values
Respiratory Distress Syndrome, Newborn / diagnosis
Specimen Handling / methods
Sphingomyelins / analysis
Surface Properties
Viscosity
Chemical
Reg. No./Substance:
0/Fatty Acids; 0/Phosphatidylcholines; 0/Phosphatidylglycerols; 0/Pulmonary Surfactants; 0/Sphingomyelins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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