Document Detail


Measurement of the absolute immature platelet number reflects marrow production and is not impacted by platelet transfusion.
MedLine Citation:
PMID:  23043309     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: The ability to distinguish increased platelet (PLT) destruction from PLT hypoproduction is important in the care of patients with marrow failure syndromes and patients receiving high-dose chemotherapy. The measurement of immature circulating PLTs based on RNA content using an automated counter is now feasible. This study evaluated the impact of recent PLT transfusion on measurement of immature PLT variables.
STUDY DESIGN AND METHODS: The immature PLT fraction (IPF) and absolute immature PLT number (AIPN) were measured using a hematology analyzer before and after PLT transfusion in nine transfusion-dependent patients with marrow failure secondary to aplastic anemia, myelodysplasia, or transplantation conditioning. IPF and AIPN were also measured serially over 5 days of storage in three plateletpheresis components collected from normal donors.
RESULTS: PLT transfusion did not significantly change the mean AIPN in transfused patients. In contrast, IPF decreased significantly from 6.6 ± 4.6% on Day -1 to 2.3 ± 1.4% on Day 0 before returning to 4.3 ± 2.3% on Day +1. In the PLT component, AIPN and IPF% increased significantly over 5 days of storage, most likely due to an artifact of the staining and detection process for stored PLTs, no longer detected in vivo once the PLTs were transfused.
CONCLUSION: PLT transfusion decreases the IPF due to the resultant increase in circulating PLT count. However, PLT transfusion does not change the circulating AIPN, validating this assay as a reflection of ongoing PLT production by the marrow in various clinical settings, regardless of proximity to PLT transfusion.
Authors:
Taha Bat; Susan F Leitman; Katherine R Calvo; Donna Chauvet; Cynthia E Dunbar
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Publication Detail:
Type:  Journal Article     Date:  2012-10-09
Journal Detail:
Title:  Transfusion     Volume:  53     ISSN:  1537-2995     ISO Abbreviation:  Transfusion     Publication Date:  2013 Jun 
Date Detail:
Created Date:  2013-06-11     Completed Date:  2013-08-12     Revised Date:  2014-06-03    
Medline Journal Info:
Nlm Unique ID:  0417360     Medline TA:  Transfusion     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1201-4     Citation Subset:  IM    
Copyright Information:
© 2012 American Association of Blood Banks.
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MeSH Terms
Descriptor/Qualifier:
Anemia, Aplastic / blood,  physiopathology,  therapy
Blood Platelets / cytology*
Bone Marrow / physiology*
Hematopoietic Stem Cell Transplantation
Hemoglobinuria, Paroxysmal / blood*,  physiopathology,  therapy*
Humans
Myelodysplastic Syndromes / blood,  physiopathology,  therapy
Platelet Count / methods
Platelet Transfusion*
Plateletpheresis
Transplantation Conditioning
Grant Support
ID/Acronym/Agency:
Z99 HL999999/HL/NHLBI NIH HHS
Comments/Corrections

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