Document Detail


Measurement of T-kinin in rat plasma using a specific radioimmunoassay.
MedLine Citation:
PMID:  1438985     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
T-kinin (Ile-Ser-Bradykinin) has been isolated only from the plasma of the rat and it is unclear whether the peptide, or its biosynthetic precursor, T-kininogen, circulates in the human. An NH2-terminally directed antiserum to T-kinin was raised in rabbits using an immunogen prepared by coupling the free -SH group of T-kinin extended from its COOH-terminus by a cysteinyl residue to an -NH2 group on human serum albumin. A radioimmunoassay was developed using this antiserum and 125I-labelled [Tyr10]T-kinin as tracer that was sensitive (least-detectable concentration 3 fmol/tube) and relatively specific for T-kinin (cross-reactivity with bradykinin and kallidin less than 1%). Treatment of rat plasma with an excess of trypsin in the presence of a kininase inhibitor generated T-kinin immunoreactivity equivalent to 455 +/- 71 pmol/ml (mean +/- S.E.M.; n = 9) and this immunoreactivity was eluted from a reversed-phase HPLC column as a single peak with the same retention time as synthetic T-kinin. In contrast, treatment of plasma from healthy human subjects (n = 8) and from patients (n = 8) with inflammation due to acute or chronic gastrointestinal disease under the same conditions did not generate any detectable T-kinin immunoreactivity. It is concluded, therefore, that T-kininogen, the biosynthetic precursor of T-kinin in the rat, is either absent from the plasma of human subjects or is present in a concentration less than 30 fmol/ml. Similarly, T-kininogen is probably not an acute phase reactant in humans.
Authors:
F O'Harte; D D Smith; S J Lanspa; J M Conlon
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Regulatory peptides     Volume:  41     ISSN:  0167-0115     ISO Abbreviation:  Regul. Pept.     Publication Date:  1992 Sep 
Date Detail:
Created Date:  1992-12-04     Completed Date:  1992-12-04     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  8100479     Medline TA:  Regul Pept     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  139-48     Citation Subset:  IM    
Affiliation:
Department of Biomedical Sciences, Creighton University Medical School, Omaha, NE 68178.
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MeSH Terms
Descriptor/Qualifier:
Adult
Amino Acid Sequence
Animals
Bradykinin / analogs & derivatives*,  blood,  chemistry
Female
Gastroenteritis / blood
Humans
Kininogens / blood
Male
Molecular Sequence Data
Radioimmunoassay / methods*
Rats
Rats, Sprague-Dawley
Species Specificity
Chemical
Reg. No./Substance:
0/Kininogens; 58-82-2/Bradykinin; 86030-63-9/T-kinin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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