| Measurement of T cell activation after 16-hr in vitro stimulation with concanavalin A. | |
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MedLine Citation:
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PMID: 20069526 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A flow cytometry assay that can be used to directly determine the proportion of activated T lymphocytes in human whole blood samples after stimulation with concanavalin A is presented here. Human whole blood is incubated with fluorescently labeled antibodies (against CD3, CD4, CD8, and CD69), erythrocytes are then lysed, and the samples are analyzed using a flow cytometer. The assay presented is able to differentiate between CD4+ and CD8+ T lymphocytes. Thus, it is possible to quantify both lymphocyte populations in parallel, as well as the respective proportions of activated T lymphocytes, all from one sample. An additional advantage of this assay is that it was developed to assay whole blood, thereby reducing the likelihood of introducing pre-analytic error and facilitating the calculation of quantitative ratios. Thus, the method can be used for both in vitro and ex vivo experiments. |
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Authors:
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Borros M Arneth |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Current protocols in cytometry / editorial board, J. Paul Robinson, managing editor ... [et al.] Volume: Chapter 6 ISSN: 1934-9300 ISO Abbreviation: Curr Protoc Cytom Publication Date: 2010 Jan |
Date Detail:
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Created Date: 2010-01-13 Completed Date: 2010-03-09 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 100899351 Medline TA: Curr Protoc Cytom Country: United States |
Other Details:
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Languages: eng Pagination: Unit 6.28.1-10 Citation Subset: IM |
Affiliation:
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Institute of Clinical Chemistry and Laboratory Medicine, Johannes Gutenberg University Mainz, Mainz, Germany. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Antibodies, Monoclonal
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pharmacology CD4-Positive T-Lymphocytes / drug effects, immunology CD8-Positive T-Lymphocytes / drug effects, immunology Cell Extracts Concanavalin A / immunology*, pharmacology Erythrocytes / drug effects Flow Cytometry / methods* Humans Immunophenotyping Lymphocyte Activation* / drug effects Lymphocyte Subsets / drug effects Time Factors |
| Chemical | |
Reg. No./Substance:
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0/Antibodies, Monoclonal; 0/Cell Extracts; 11028-71-0/Concanavalin A |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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