| Measurement of S-methylcysteine and S-methyl-mercapturic acid in human urine by alkyl-chloroformate extractive derivatization and isotope-dilution gas chromatography-mass spectrometry. | |
| | |
MedLine Citation:
|
PMID: 21110385 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
|
S-methylcysteine (SMC) is a minor amino acid naturally excreted in human urine, a protective agent against oxidative stress and a biotransformation product of the fumigant biocide methyl bromide and of nicotine. A metabolic source of SMC is catabolism of the repair catalytic protein MGMT (EC 2.1.1.37), which specifically removes the methyl group from the modified DNA nucleotide O-6-methyl-guanine to revert the normal GC base pairing. To assess the value of SMC and of S-methylmercapturic acid (SMMA) as candidate biomarkers of proliferative phenomena, a sensitive analytical method by GC-MS was applied in a pilot study of healthy subjects to assess their urinary elimination and the intra- and inter-individual variability. Extractive alkylation with butylchloroformate-n-butanol-pyridine (Husek technique) was employed for sample derivatization and isotope dilution GC-MS with S-[CD(3) ]-SMC and -SMMA was applied for specific and sensitive detection. To resolve the target analytes from the main coeluting interferents in the derivatized urine extract a medium-polarity stationary phase was employed. SMMA was not detected in the morning urine of three healthy fertile-age women followed for one month above the minimum detectable level of approx. 500 µg/L while SMC concentrations were in the 0.02-0.7 µg/mL range (n = 61) with large inter-day and inter-individual variations. In a young healthy male urine samples taken throughout a few days yielded concentrations in the same 90-810 µg/L range (n = 11). These preliminary results points at SMC as a candidate biomarker for the study of methylation turnover in several biochemical processes. Copyright © 2010 John Wiley & Sons, Ltd. |
| | |
Authors:
|
Federico M Rubino; Marco Pitton; Daniela Di Fabio; Giuseppe Meroni; Enzo Santaniello; Enrico Caneva; Marco Pappini; Antonio Colombi |
Related Documents
:
|
19863875 - Glomerular filtration rate measurements by 125i-iothalamate should be corrected for ina... 5602575 - Determination of iron in urine with special reference to the desferrioxamine test. 9800385 - Study on urine boron reference values of japanese men: use of confidence intervals as a... 2456405 - Characterization of urinary keratin number 18 using a new assay. 21862025 - Separation of exenatide analogue mono-pegylated with 40kda polyethylene glycol by catio... 10396815 - Sensitive determination of paraquat and diquat at the sub-ng ml-1 level by continuous a... |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: Biomedical chromatography : BMC Volume: 25 ISSN: 1099-0801 ISO Abbreviation: Biomed. Chromatogr. Publication Date: 2011 Mar |
Date Detail:
|
Created Date: 2011-02-15 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 8610241 Medline TA: Biomed Chromatogr Country: England |
Other Details:
|
Languages: eng Pagination: 330-43 Citation Subset: IM |
Copyright Information:
|
Copyright © 2010 John Wiley & Sons, Ltd. |
Affiliation:
|
LaTMA Laboratory for Analytical Toxicology and Metabonomics, Department of Medicine, Surgery and Dental Sciences, Università degli Studi di Milano at 'Ospedale San Paolo', v. A di Rudinì 8, Milano I-20142, Italy. Federico.Rubino@unimi.it. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: The validation of a simple LC/MS/MS method for determining the level of mevalonic acid in human plas...
Next Document: Identification of major metabolites in rat urine and plasma of N(6) -(4-hydroxybenzyl) adenine ribos...