Document Detail


Measurement of Reference Intervals for Urinary Free Adrenal Steroid Levels in Japanese Newborn Infants by Using Stable Isotope Dilution Gas Chromatography/Mass Spectrometry.
MedLine Citation:
PMID:  23159298     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
BACKGROUND: In newborn infants, there are no reference intervals for urinary free steroids, which are thought to reflect the bioavailable fraction of steroids in the blood. We establish a method for simultaneous measurement of urinary free adrenal steroids such as pregnenolone, progesterone, 16α-hydroxyprogesterone, 17α-hydroxyprogesterone, 21-deoxycortisone, 21-deoxycortisol, dehydroepiandrosterone, androstenedione, and 11β-hydroxyandrostenedione by using stable isotope dilution gas chromatography/mass spectrometry (SID-GC/MS) and determined the reference intervals for urinary levels of free adrenal steroids in Japanese newborn infants. METHODS: Newborn pooled urine was used for validation. Spot urine samples were collected from 67 full-term Japanese newborn infants (34 male and 33 female infants) at 3-4days of age to determine reference intervals. The extracted and purified free steroids were delivered with heptafluorobutyric anhydride and analyzed by SID-GC/MS. RESULTS: We validated a SID-GC/MS method with good repeatability and recovery rate. The preliminary reference intervals (median [range], μmol/mol creatinine) were as follows: pregnenolone, 4.2 (0.7-31.6); progesterone, 0.5 (not detected (n.d.)-0.6); 16α-hydroxyprogesterone, 1.4 (n.d.-10.3); 17α-hydroxyprogesterone, 1.1 (n.d.-1.9); 21-deoxycortisone, n.d. (n.d.-n.d.); 21-deoxycortisol, n.d. (n.d.-n.d.); dehydroepiandrosterone, 2.2 (0.6-27.3); androstenedione, 0.7 (n.d.-5.2); and 11β-hydroxyandrostenedione, 2.9 (n.d.-26.7). CONCLUSIONS: We established a reliable SID-GC/MS method and were able to determine preliminary reference intervals for 9 urinary free adrenal steroids in newborn infants.
Authors:
Yuhei Koyama; Keiko Homma; Masayuki Miwa; Kazushige Ikeda; Mitsuru Murata; Tomonobu Hasegawa
Related Documents :
15830058 - Prognostic value of non-reactive burst suppression eeg pattern associated to early neon...
12907278 - Neonatal seizures: characteristics of eeg ictal activity in preterm and fullterm infants.
3511258 - Optic nerve hypoplasia in infancy.
23154158 - Newborn screening for congenital hypothyroidism.
2658548 - Evaluation of the vomiting infant.
22480138 - The live-birth prevalence of mucopolysaccharidoses in estonia.
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-11-15
Journal Detail:
Title:  Clinica chimica acta; international journal of clinical chemistry     Volume:  -     ISSN:  1873-3492     ISO Abbreviation:  Clin. Chim. Acta     Publication Date:  2012 Nov 
Date Detail:
Created Date:  2012-11-19     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  1302422     Medline TA:  Clin Chim Acta     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2012. Published by Elsevier B.V.
Affiliation:
Department of Laboratory Medicine, Keio University School of Medicine, Tokyo 160-8582, Japan; Mitsubishi Chemical Medience Co., Tokyo 108-8559, Japan.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Limitations in Qualitative Point of Care hCG Tests for Detecting Early Pregnancy.
Next Document:  Design and implementation of software for automated quality control and data analysis for a complex ...