Document Detail


Measurement of plasma volume using fluorescent silica-based nanoparticles.
MedLine Citation:
PMID:  22174395     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Plasma volume (PV) is an important determinant of cardiovascular function and organ perfusion, and it is the target of infusion and diuretic therapies in daily clinical practice. Despite its fundamental importance PV is not commonly measured because available methods of tracer dilution are reliant on dye substances that suffer from numerous drawbacks including binding plasma proteins, spectral changes, and clearance kinetics that complicate analysis and interpretation. To address these issues, we have tested the utility of fluorescent nanoparticles comprised of a dye-rich silica core and polyethylene glycol-coated shell. Photophysical and visual analysis showed discrete size-gradated nanoparticle populations could be synthesized within a distribution tolerance of ±4 nm, which were optically unaffected in the presence of plasma/albumin. In normal mice, the cutoff for renal filtration of nanoparticles from blood into urine was ≤11 nm. A linear relationship between body weight and PV was readily determined in mice administered far red fluorescent nanoparticles sized either 20 or 30 nm. PV measurements using nanoparticles were correlated to values obtained with Evans blue dye. Induced expansion or contraction of PV was demonstrated with albumin or furosemide administration, respectively, in mice. Longitudinal experiments >30 min required matched untreated control mice to correct for nanoparticle loss (≈30%) putatively to the reticuloendothelial/phagocyte system. Collectively, the findings support a nanotechnology-based solution to methodological problems in measure of PV, notably in clinical settings where information on hemodynamic changes may improve treatment of injury and disease.
Authors:
Christoph Eisner; Hooisweng Ow; Tianxin Yang; Zhanjun Jia; Emilios Dimitriadis; Lingli Li; Kenneth Wang; Josephine Briggs; Mark Levine; Jurgen Schnermann; Michael Graham Espey
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Intramural     Date:  2011-12-15
Journal Detail:
Title:  Journal of applied physiology (Bethesda, Md. : 1985)     Volume:  112     ISSN:  1522-1601     ISO Abbreviation:  J. Appl. Physiol.     Publication Date:  2012 Feb 
Date Detail:
Created Date:  2012-02-16     Completed Date:  2012-06-22     Revised Date:  2013-09-26    
Medline Journal Info:
Nlm Unique ID:  8502536     Medline TA:  J Appl Physiol (1985)     Country:  United States    
Other Details:
Languages:  eng     Pagination:  681-7     Citation Subset:  IM    
Affiliation:
National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Blood Proteins / chemistry
Fluorescent Dyes / diagnostic use*
Kidney / physiology
Male
Mice
Mice, Inbred C57BL
Microscopy, Atomic Force
Nanoparticles / diagnostic use*
Plasma Volume*
Polyethylene Glycols
Silicon Dioxide
Spectrometry, Fluorescence
Chemical
Reg. No./Substance:
0/Blood Proteins; 0/Fluorescent Dyes; 0/Polyethylene Glycols; 7631-86-9/Silicon Dioxide
Comments/Corrections

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