Document Detail


Measurement of DNA copy number at microsatellite loci using quantitative PCR analysis.
MedLine Citation:
PMID:  11034080     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
This report describes the development and validation of quantitative microsatellite analysis (QuMA) for rapid measurement of relative DNA sequence copy number. In QuMA, the copy number of a test locus relative to a pooled reference is assessed using quantitative, real-time PCR amplification of loci carrying simple sequence repeats. Use of simple sequence repeats is advantageous because of the large numbers that are mapped precisely. In addition, all markers are informative because QuMA does not require that they be polymorphic. The utility of QuMA is demonstrated in assessment of the extent of deletions of chromosome 2 in leukemias arising in radiation-sensitive inbred SJL mice and in analysis of the association of increased copy number of the putative oncogene ZNF217 with reduced survival duration in ovarian cancer patients.
Authors:
D G Ginzinger; T E Godfrey; J Nigro; D H Moore; S Suzuki; M G Pallavicini; J W Gray; R H Jensen
Publication Detail:
Type:  Journal Article; Validation Studies    
Journal Detail:
Title:  Cancer research     Volume:  60     ISSN:  0008-5472     ISO Abbreviation:  Cancer Res.     Publication Date:  2000 Oct 
Date Detail:
Created Date:  2000-10-16     Completed Date:  2000-10-27     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  2984705R     Medline TA:  Cancer Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  5405-9     Citation Subset:  IM    
Affiliation:
Department of Laboratory Medicine, University of California San Francisco Comprehensive Cancer Center, University of California, 94143, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
DNA, Neoplasm / analysis,  genetics*
Female
Gene Dosage*
Genes, Tumor Suppressor / genetics
Humans
Leukemia, Myeloid / etiology,  genetics
Leukemia, Radiation-Induced / genetics
Male
Mice
Mice, Inbred Strains
Mice, Knockout
Microsatellite Repeats / genetics*
Neoplasm Proteins / genetics
Nucleic Acid Amplification Techniques
Ovarian Neoplasms / genetics
Polymerase Chain Reaction / methods*
Prognosis
Reproducibility of Results
Survival Analysis
Trans-Activators / genetics
Chemical
Reg. No./Substance:
0/DNA, Neoplasm; 0/Neoplasm Proteins; 0/Trans-Activators; 0/ZNF217 protein, human

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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