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Measurement of Chemosensitivity and Growth Rate in p53 Expressing Cells.
MedLine Citation:
PMID:  23150442     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Chemoresistance and increased growth rate are two gain-of-function functions that mutant p53 is thought to possess. Here, we describe two methods for measuring the sensitiveness of cells to chemotherapeutic drugs and the rate of cell growth. Both of which can be used with a wide range of cell types. The clonogenic assay can be used with many different chemotoxic drugs and the growth assay described here presents an alternative to the MTT assay and allows for a long-term measurement of cell growth. These protocols are both easy, flexible, require relatively little effort, and are inexpensive to carry out.
Authors:
Mahesh Ramamoorthy; Catherine Vaughan; Sumitra Deb; Swati Palit Deb
Publication Detail:
Type:  JOURNAL ARTICLE    
Journal Detail:
Title:  Methods in molecular biology (Clifton, N.J.)     Volume:  962     ISSN:  1940-6029     ISO Abbreviation:  Methods Mol. Biol.     Publication Date:  2013  
Date Detail:
Created Date:  2012-11-14     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9214969     Medline TA:  Methods Mol Biol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  127-133     Citation Subset:  -    
Affiliation:
Laboratory of Molecular Gerontology, National Institute on Aging, Biomedical Research Center, Baltimore, MD, USA.
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