Document Detail

Measurement of Caspase Activity: From Cell Populations to Individual Cells.
MedLine Citation:
PMID:  21468969     Owner:  NLM     Status:  Publisher    
Caspases are critical regulators of the apoptotic program, responsible for the harmonic dismantling of the cell. Cell death can occur by way of different options (necroptosis, necrosis, extreme autophagy) but once caspases are fully engaged it will take the apoptotic route. Hence, in general, caspase activity is inversely proportional to cell viability. Caspase activation can be measured by means of different techniques. Here, we describe three different methodologies to measure/observe caspase activation. Two approaches are recommended for studies on the whole cell population, whereas the third one is designed for visualizing caspase activation in single live cells.
Gabriela Paroni; Claudio Brancolini
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Publication Detail:
Journal Detail:
Title:  Methods in molecular biology (Clifton, N.J.)     Volume:  740     ISSN:  1940-6029     ISO Abbreviation:  -     Publication Date:  2011  
Date Detail:
Created Date:  2011-4-6     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9214969     Medline TA:  Methods Mol Biol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  65-79     Citation Subset:  -    
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