Document Detail

Measurement of Caspase Activity: From Cell Populations to Individual Cells.
MedLine Citation:
PMID:  21468969     Owner:  NLM     Status:  Publisher    
Caspases are critical regulators of the apoptotic program, responsible for the harmonic dismantling of the cell. Cell death can occur by way of different options (necroptosis, necrosis, extreme autophagy) but once caspases are fully engaged it will take the apoptotic route. Hence, in general, caspase activity is inversely proportional to cell viability. Caspase activation can be measured by means of different techniques. Here, we describe three different methodologies to measure/observe caspase activation. Two approaches are recommended for studies on the whole cell population, whereas the third one is designed for visualizing caspase activation in single live cells.
Gabriela Paroni; Claudio Brancolini
Related Documents :
25176449 - Leishmania amazonensis: increase in ecto-atpase activity and parasite burden of vinblas...
18286619 - Boronate-containing polymer brushes: characterization, interaction with saccharides and...
21422199 - Metformin induces both caspase-dependent and poly(adp-ribose) polymerase-dependent cell...
3518959 - Polymeric microspheres as diagnostic tools for cell surface marker tracing.
19872519 - Dissimilarity of inner and outer protoplasmic surfaces in valonia. ii.
18287659 - Mammalian lectin as tool in glycochemistry and histochemistry with relevance for diagno...
109329 - Hormonal regulation in two rat mammary cancer cell lines: glucocorticoid and androgen r...
20118219 - Temporal relationship between primary and motile ciliogenesis in airway epithelial cells.
429379 - A method for toxicity screening of biomaterials using cells cultured on millipore filters.
Publication Detail:
Journal Detail:
Title:  Methods in molecular biology (Clifton, N.J.)     Volume:  740     ISSN:  1940-6029     ISO Abbreviation:  -     Publication Date:  2011  
Date Detail:
Created Date:  2011-4-6     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9214969     Medline TA:  Methods Mol Biol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  65-79     Citation Subset:  -    
, .
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Annexin V/7-AAD Staining in Keratinocytes.
Next Document:  Rapid quantification of cell viability and apoptosis in B-cell lymphoma cultures using cyanine SYTO ...