| Measurement of 3-nitro-tyrosine in human plasma and urine by gas chromatography-tandem mass spectrometry. | |
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MedLine Citation:
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PMID: 22125150 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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Reaction of reactive nitrogen species (RNS), such as peroxynitrite and nitryl chloride with soluble tyrosine and tyrosine residues in proteins produces soluble 3-nitro-tyrosine and 3-nitro-tyrosino-proteins, respectively. Regular proteolysis of 3-nitro-tyrosino-proteins yields soluble 3-nitro-tyrosine. 3-Nitro-tyrosine circulates in plasma and is excreted in the urine. Both circulating and excretory 3-nitro-tyrosine are considered suitable biomarkers of nitrative stress. Tandem mass spectrometry coupled with gas chromatography (GC-MS/MS) or liquid chromatography (LC-MS/MS) is one of the most reliable analytical techniques to determine 3-nitro-tyrosine. Here, we describe protocols for the quantitative determination of soluble 3-nitro-tyrosine in human plasma and urine by GC-MS/MS. |
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Authors:
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Dimitrios Tsikas; Anja Mitschke; Frank-Mathias Gutzki |
Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Methods in molecular biology (Clifton, N.J.) Volume: 828 ISSN: 1940-6029 ISO Abbreviation: Methods Mol. Biol. Publication Date: 2012 |
Date Detail:
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Created Date: 2011-11-29 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9214969 Medline TA: Methods Mol Biol Country: United States |
Other Details:
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Languages: eng Pagination: 255-70 Citation Subset: IM |
Affiliation:
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Institute of Clinical Pharmacology, Hannover Medical School, Hannover, Germany, tsikas.dimitros@mh-hannover.de. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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