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Measurement of [(18)F]-fluorodeoxyglucose incorporation into human osteoblast-An experimental method.
MedLine Citation:
PMID:  19003012     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
An evaluation of human osteoblast metabolism usually involves measurements of the by-products of bone matrix elaboration. The assessment of glycolytic activity of osteoblasts is not a standard tool in most of the reports, but might be of value by providing a direct indicator of cellular metabolism. Measurement of the incorporation of [(18)F]-fluorodeoxyglucose, which is not further degradable following its conversion into glycose-6-phosphate during glycolysis and is trapped in this form within the cells, can be used as an effective research tool for estimation of osteoblast metabolism. In order to estimate the [(18)F]-fluorodeoxyglucose incorporation we used cultured human osteoblast-like cells. Following incubation of the culture samples in a glucose free medium with 5 mu Ci [(18)F]-fluorodeoxyglucose we measured the radioactivity of the cell fraction, as a percent from the initial dose, and compared to the incorporation values in cells treated by protoporphyrine IX (10(-5) M), an endogenous pro-apoptotic agent. To compare the response of [(18)F]-fluorodeoxyglucose incorporation studies, following treatment of cells with the protoporphyrine IX, to other experimental cell metabolism evaluation methods, we performed a parallel comparison of alkaline phospatase activity, which is a standard measurement tool of osteoblast metabolism, in the control and treatment groups. A narrow range of 0.22-1.36% of [(18)F]-fluorodeoxyglucose incorporation per million cells was found. Additionally in the protoporphyrine IX treated cells a significant 62% decrease of [(18)F]-fluorodeoxyglucose incorporation was observed (p < .05). A parallel significant decrease in alkaline phosphatase activity (p < .001) was found in the cells treated by the protoporphyrine IX. Therefore we suggest that the presented method of [(18)F]-fluorodeoxyglucose incorporation measurement can be utilized as an effective research tool for estimation of the cellular glycolitic activity in human osteoblast-like cells in vitro.
Authors:
Nahum Rosenberg; Lise Bettman; Orit Rosenberg; Michael Soudry; Moshe Gavish; Rachel Bar-Shalom
Publication Detail:
Type:  Journal Article     Date:  2007-04-24
Journal Detail:
Title:  Cytotechnology     Volume:  54     ISSN:  0920-9069     ISO Abbreviation:  Cytotechnology     Publication Date:  2007 May 
Date Detail:
Created Date:  2008-11-12     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8807027     Medline TA:  Cytotechnology     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  1-4     Citation Subset:  -    
Affiliation:
Department of Orthopaedic Surgery "A", Rambam Medical Center, P.O. 9602, Haifa, 31096, Israel, nahumrosenberg@hotmail.com.
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