Document Detail


Mcl-1 is essential for germinal center formation and B cell memory.
MedLine Citation:
PMID:  20929728     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Lymphocyte survival during immune responses is controlled by the relative expression of pro- and anti-apoptotic molecules, regulating the magnitude, quality, and duration of the response. We investigated the consequences of deleting genes encoding the anti-apoptotic molecules Mcl1 and Bcl2l1 (Bcl-x(L)) from B cells using an inducible system synchronized with expression of activation-induced cytidine deaminase (Aicda) after immunization. This revealed Mcl1 and not Bcl2l1 to be indispensable for the formation and persistence of germinal centers (GCs). Limiting Mcl1 expression reduced the magnitude of the GC response with an equivalent, but not greater, effect on memory B cell formation and no effect on persistence. Our results identify Mcl1 as the main anti-apoptotic regulator of activated B cell survival and suggest distinct mechanisms controlling survival of GC and memory B cells.
Authors:
Ingela Vikstrom; Sebastian Carotta; Katja Lüthje; Victor Peperzak; Philipp J Jost; Stefan Glaser; Meinrad Busslinger; Philippe Bouillet; Andreas Strasser; Stephen L Nutt; David M Tarlinton
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2010-10-07
Journal Detail:
Title:  Science (New York, N.Y.)     Volume:  330     ISSN:  1095-9203     ISO Abbreviation:  Science     Publication Date:  2010 Nov 
Date Detail:
Created Date:  2010-11-24     Completed Date:  2010-12-14     Revised Date:  2011-09-26    
Medline Journal Info:
Nlm Unique ID:  0404511     Medline TA:  Science     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1095-9     Citation Subset:  IM    
Affiliation:
Walter and Eliza Hall Institute of Medical Research, 1G Royal Parade, Parkville, Victoria 3052, Australia.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/HM804028;  HM804029;  HM804030;  HM804031;  HM804032;  HM804033;  HM804034;  HM804035;  HM804036;  HM804037;  HM804038;  HM804039;  HM804040;  HM804041;  HM804042;  HM804043;  HM804044;  HM804045;  HM804046;  HM804047;  HM804048;  HM804049;  HM804050;  HM804051;  HM804052;  HM804053;  HM804054;  HM804055;  HM804056;  HM804057;  HM804058;  HM804059;  HM804060;  HM804061;  HM804062;  HM804063;  HM804064;  HM804065;  HM804066;  HM804067;  HM804068;  HM804069;  HM804070;  HM804071;  HM804072;  HM804073;  HM804074;  HM804075;  HM804076;  HM804077;  HM804078;  HM804079;  HM804080;  HM804081;  HM804082;  HM804083;  HM804084
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MeSH Terms
Descriptor/Qualifier:
Animals
Antibody Affinity
B-Lymphocytes / immunology*
Cell Survival
Gene Deletion
Germinal Center / cytology,  immunology*
Immunologic Memory*
Mice
Mice, Inbred C57BL
Molecular Sequence Data
Proto-Oncogene Proteins c-bcl-2 / genetics,  immunology*
bcl-X Protein / genetics,  immunology
Grant Support
ID/Acronym/Agency:
CA43540/CA/NCI NIH HHS; CA80188/CA/NCI NIH HHS; R01 CA043540-22/CA/NCI NIH HHS; R01 CA080188-08/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
0/Proto-Oncogene Proteins c-bcl-2; 0/bcl-X Protein; 0/myeloid cell leukemia sequence 1 protein

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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