| Mature interleukin-33 is produced by calpain-mediated cleavage in vivo. | |
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MedLine Citation:
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PMID: 19596270 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Interleukin (IL)-33 is a novel member of the IL-1 family. IL-33 is primarily synthesized as a 30-kDa precursor (pro-IL-33). Pro-IL-33 is cleaved by caspase-1 into an 18-kDa mature form (mature IL-33) in vitro. Recombinant mature IL-33 has been known to induce T-helper type-2 (Th2)-associated cytokines and inflammatory cytokines via its receptor, ST2L. However, processing of pro-IL-33 in vivo has not been clarified yet. Here, we report that calpain mediates pro-IL-33 processing in vivo. Pro-IL-33 was expressed by stimulating human epithelial cells with phorbol 12-myristate 13-acetate. Calcium ionophore induced pro-IL-33 cleavage and mature IL-33 production. This cleavage was inhibited by treatment with a calcium chelator and calpain inhibitors. Moreover, short interfering RNA-mediated knockdown of calpains suppressed pro-IL-33 cleavage. These results indicate that calpains play a critical role in pro-IL-33 processing in vivo. |
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Authors:
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Morisada Hayakawa; Hiroko Hayakawa; Yasushi Matsuyama; Hiroyuki Tamemoto; Hitoaki Okazaki; Shin-ichi Tominaga |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-07-09 |
Journal Detail:
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Title: Biochemical and biophysical research communications Volume: 387 ISSN: 1090-2104 ISO Abbreviation: Biochem. Biophys. Res. Commun. Publication Date: 2009 Sep |
Date Detail:
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Created Date: 2009-07-31 Completed Date: 2009-08-14 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0372516 Medline TA: Biochem Biophys Res Commun Country: United States |
Other Details:
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Languages: eng Pagination: 218-22 Citation Subset: IM |
Affiliation:
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Department of Biochemistry, Jichi Medical University, 3311-1 Yakushiji, Shimotsuke-shi, Tochigi, Japan. morisada@jichi.ac.jp |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Calcium
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pharmacology Calpain / genetics, metabolism* Cell Line, Tumor Endothelial Cells / drug effects, enzymology, metabolism Epithelial Cells / drug effects, enzymology, metabolism Humans Interleukins / metabolism* RNA, Small Interfering / genetics Tetradecanoylphorbol Acetate / pharmacology |
| Chemical | |
Reg. No./Substance:
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0/IL33 protein, human; 0/Interleukins; 0/RNA, Small Interfering; 16561-29-8/Tetradecanoylphorbol Acetate; 7440-70-2/Calcium; EC 3.4.22.-/Calpain |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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