Document Detail


Mature adipocyte-derived dedifferentiated fat cells can transdifferentiate into skeletal myocytes in vitro.
MedLine Citation:
PMID:  18938140     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We have previously reported the establishment of preadipocyte cell lines, termed dedifferentiated fat (DFAT) cells, from mature adipocytes of various animals. DFAT cells possess long-term viability and can redifferentiate into adipocytes both in vivo and in vitro. Furthermore, DFAT cells can transdifferentiate into osteoblasts and chondrocytes under appropriate culture conditions. However, it is unclear whether DFAT cells are capable of transdifferentiating into skeletal myocytes, which is common in the mesodermal lineage. Here, we show that DFAT cells can be induced to transdifferentiate into skeletal myocytes in vitro. Myogenic induction of DFAT cells resulted in the expression of MyoD and myogenin, followed by cell fusion and formation of multinucleated cells expressing sarcomeric myosin heavy chain. These results indicate that DFAT cells derived from mature adipocytes can transdifferentiate into skeletal myocytes in vitro.
Authors:
Tomohiko Kazama; Masaki Fujie; Tuyoshi Endo; Koichiro Kano
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-10-18
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  377     ISSN:  1090-2104     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  2008 Dec 
Date Detail:
Created Date:  2008-11-25     Completed Date:  2008-12-15     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  United States    
Other Details:
Languages:  eng     Pagination:  780-5     Citation Subset:  IM    
Affiliation:
Laboratory of Cell and Tissue Biology, Graduate School of Bioresource Sciences, Nihon University, 1866 Kameino, Fujisawa, Kanagawa 252-8510, Japan.
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MeSH Terms
Descriptor/Qualifier:
Adipocytes / cytology*,  drug effects,  metabolism
Adipogenesis
Animals
Azacitidine / pharmacology
Base Sequence
Cell Line
Cell Lineage
Cell Transdifferentiation* / genetics
DNA Methylation / drug effects
Gene Silencing
Mice
Molecular Sequence Data
Muscle Fibers, Skeletal / cytology*
MyoD Protein / genetics
Promoter Regions, Genetic
Reverse Transcriptase Polymerase Chain Reaction
Chemical
Reg. No./Substance:
0/MyoD Protein; 0/MyoD1 myogenic differentiation protein; 320-67-2/Azacitidine

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