| Mature adipocyte-derived dedifferentiated fat cells can transdifferentiate into skeletal myocytes in vitro. | |
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MedLine Citation:
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PMID: 18938140 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We have previously reported the establishment of preadipocyte cell lines, termed dedifferentiated fat (DFAT) cells, from mature adipocytes of various animals. DFAT cells possess long-term viability and can redifferentiate into adipocytes both in vivo and in vitro. Furthermore, DFAT cells can transdifferentiate into osteoblasts and chondrocytes under appropriate culture conditions. However, it is unclear whether DFAT cells are capable of transdifferentiating into skeletal myocytes, which is common in the mesodermal lineage. Here, we show that DFAT cells can be induced to transdifferentiate into skeletal myocytes in vitro. Myogenic induction of DFAT cells resulted in the expression of MyoD and myogenin, followed by cell fusion and formation of multinucleated cells expressing sarcomeric myosin heavy chain. These results indicate that DFAT cells derived from mature adipocytes can transdifferentiate into skeletal myocytes in vitro. |
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Authors:
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Tomohiko Kazama; Masaki Fujie; Tuyoshi Endo; Koichiro Kano |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2008-10-18 |
Journal Detail:
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Title: Biochemical and biophysical research communications Volume: 377 ISSN: 1090-2104 ISO Abbreviation: Biochem. Biophys. Res. Commun. Publication Date: 2008 Dec |
Date Detail:
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Created Date: 2008-11-25 Completed Date: 2008-12-15 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0372516 Medline TA: Biochem Biophys Res Commun Country: United States |
Other Details:
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Languages: eng Pagination: 780-5 Citation Subset: IM |
Affiliation:
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Laboratory of Cell and Tissue Biology, Graduate School of Bioresource Sciences, Nihon University, 1866 Kameino, Fujisawa, Kanagawa 252-8510, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adipocytes
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cytology*,
drug effects,
metabolism Adipogenesis Animals Azacitidine / pharmacology Base Sequence Cell Line Cell Lineage Cell Transdifferentiation* / genetics DNA Methylation / drug effects Gene Silencing Mice Molecular Sequence Data Muscle Fibers, Skeletal / cytology* MyoD Protein / genetics Promoter Regions, Genetic Reverse Transcriptase Polymerase Chain Reaction |
| Chemical | |
Reg. No./Substance:
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0/MyoD Protein; 0/MyoD1 myogenic differentiation protein; 320-67-2/Azacitidine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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