Document Detail


Maturation of sperm volume regulation in the rat epididymis.
MedLine Citation:
PMID:  20531277     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Sperm maturation in the epididymis may involve differences between mature and immature spermatozoa in their volume regulatory osmolyte response. Spermatozoa obtained from the rat caput and cauda epididymidis were examined for their ability to regulate volume after transfer from in situ epididymal osmolality (measured to be 343 +/- 13 and 365 +/- 19 mmol kg(-1), respectively) to that of the female tract in single- and multiple-step protocols. Cells withstood the single-step treatment better than the multistep protocol. Sperm volume estimates by flow cytometric measurements of forward scatter of cells with intact head membranes was more sensitive than those by assessing cell coiling microscopically. At osmolalites below 210 mmol kg(-1) both caput and cauda cells ruptured, limiting the use of flow cytometry. Above this critical value, the use of quinine showed that both caput and cauda cells could regulate volume, but cauda cells were the more effective. Of several organic osmolytes studied, myo-inositol, glutamate and KCl caused only temporary and slight swelling of spermatozoa cells in hypotonic medium. Spermatozoa of both maturities seemed to use potassium as the preferred osmolyte for regulating volume.
Authors:
Oliver S Damm; Trevor G Cooper
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-06-07
Journal Detail:
Title:  Asian journal of andrology     Volume:  12     ISSN:  1745-7262     ISO Abbreviation:  Asian J. Androl.     Publication Date:  2010 Jul 
Date Detail:
Created Date:  2010-07-05     Completed Date:  2010-10-04     Revised Date:  2013-09-17    
Medline Journal Info:
Nlm Unique ID:  100942132     Medline TA:  Asian J Androl     Country:  China    
Other Details:
Languages:  eng     Pagination:  578-90     Citation Subset:  IM    
Affiliation:
Centre of Reproductive Medicine and Andrology of the University Münster, Münster, Germany.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Size / drug effects*
Epididymis / cytology,  physiology
Female
Flow Cytometry
Glutamic Acid / pharmacology
Inositol / pharmacology
Male
Osmolar Concentration
Potassium Chloride / pharmacology
Rats
Rats, Sprague-Dawley
Sperm Maturation / drug effects,  physiology*
Spermatozoa / physiology*
Chemical
Reg. No./Substance:
56-86-0/Glutamic Acid; 6917-35-7/Inositol; 7447-40-7/Potassium Chloride
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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