Document Detail

Maturation of sperm volume regulation in the rat epididymis.
MedLine Citation:
PMID:  20531277     Owner:  NLM     Status:  MEDLINE    
Sperm maturation in the epididymis may involve differences between mature and immature spermatozoa in their volume regulatory osmolyte response. Spermatozoa obtained from the rat caput and cauda epididymidis were examined for their ability to regulate volume after transfer from in situ epididymal osmolality (measured to be 343 +/- 13 and 365 +/- 19 mmol kg(-1), respectively) to that of the female tract in single- and multiple-step protocols. Cells withstood the single-step treatment better than the multistep protocol. Sperm volume estimates by flow cytometric measurements of forward scatter of cells with intact head membranes was more sensitive than those by assessing cell coiling microscopically. At osmolalites below 210 mmol kg(-1) both caput and cauda cells ruptured, limiting the use of flow cytometry. Above this critical value, the use of quinine showed that both caput and cauda cells could regulate volume, but cauda cells were the more effective. Of several organic osmolytes studied, myo-inositol, glutamate and KCl caused only temporary and slight swelling of spermatozoa cells in hypotonic medium. Spermatozoa of both maturities seemed to use potassium as the preferred osmolyte for regulating volume.
Oliver S Damm; Trevor G Cooper
Related Documents :
17065807 - Sensors and signal transduction pathways in vertebrate cell volume regulation.
12215517 - Oscillatory chloride efflux at the pollen tube apex has a role in growth and cell volum...
3112407 - Fluorescent measurements of intracellular free calcium in isolated toad urinary bladder...
2928347 - Steroid hormones: modulators of na+ absorption and cl- secretion in cultured tracheal e...
10753937 - Proliferation of intimal smooth muscle cells. attenuation of basic fibroblast growth fa...
3519067 - Bacteriolytic factor in the salivary glands of aedes aegypti.
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-06-07
Journal Detail:
Title:  Asian journal of andrology     Volume:  12     ISSN:  1745-7262     ISO Abbreviation:  Asian J. Androl.     Publication Date:  2010 Jul 
Date Detail:
Created Date:  2010-07-05     Completed Date:  2010-10-04     Revised Date:  2013-09-17    
Medline Journal Info:
Nlm Unique ID:  100942132     Medline TA:  Asian J Androl     Country:  China    
Other Details:
Languages:  eng     Pagination:  578-90     Citation Subset:  IM    
Centre of Reproductive Medicine and Andrology of the University Münster, Münster, Germany.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Cell Size / drug effects*
Epididymis / cytology,  physiology
Flow Cytometry
Glutamic Acid / pharmacology
Inositol / pharmacology
Osmolar Concentration
Potassium Chloride / pharmacology
Rats, Sprague-Dawley
Sperm Maturation / drug effects,  physiology*
Spermatozoa / physiology*
Reg. No./Substance:
56-86-0/Glutamic Acid; 6917-35-7/Inositol; 7447-40-7/Potassium Chloride

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  The ecological coherence of high bacterial taxonomic ranks.
Next Document:  Whole-body heat exposure induces membrane changes in spermatozoa from the cauda epididymidis of labo...