| Maturation, proliferation and apoptosis of seminal tubule cells at puberty after administration of estradiol, follicle stimulating hormone or both. | |
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MedLine Citation:
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PMID: 18097524 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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AIM: To assess proliferative and apoptotic potential of the seminiferous epithelium cells in relation to Sertoli cell maturation in newborn rats under the influence of estradiol, follicle stimulating hormone (FSH) or both agents given together. METHODS: From postnatal day (PND) 5 to 15 male rats were daily injected with 12.5 microg of 17beta-estradiol benzoate (EB) or 7.5 IU of human purified FSH (hFSH) or EB + hFSH or solvents (control). On postnatal day 16, autopsy was performed. Sertoli cell maturation/function was assessed by morphometry. Proliferation of the seminiferous epithelium cells was quantitatively evaluated using immunohistochemical labeling against proliferating cell nuclear antigen and apoptosis using the TUNEL method. RESULTS: Although EB inhibited Sertoli cell maturation and hFSH was not effective, a pronounced acceleration of Sertoli cell maturation occurred after EB + hFSH. Whereas hFSH stimulated Sertoli cell proliferation, EB or EB + hFSH inhibited Sertoli cell proliferation. All treatments significantly stimulated germ cell proliferation. Apoptosis of Sertoli cells increased 9-fold and germ cells 2-fold after EB, and was not affected by hFSH but was inhibited after EB + hFSH. CONCLUSION: At puberty, estradiol inhibits Sertoli cell maturation, increases Sertoli and germ cell apoptosis but stimulates germ cell proliferation. Estradiol in synergism with FSH, but neither of the hormones alone, accelerates Sertoli cell maturation associated with an increase in germ cell survival. Estradiol and FSH cooperate to induce seminal tubule maturation and trigger first spermatogenesis. |
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Authors:
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Renata Walczak-Jedrzejowska; Jolanta Slowikowska-Hilczer; Katarzyna Marchlewska; Krzysztof Kula |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2007-12-20 |
Journal Detail:
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Title: Asian journal of andrology Volume: 10 ISSN: 1008-682X ISO Abbreviation: Asian J. Androl. Publication Date: 2008 Jul |
Date Detail:
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Created Date: 2008-05-14 Completed Date: 2008-07-29 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 100942132 Medline TA: Asian J Androl Country: China |
Other Details:
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Languages: eng Pagination: 585-92 Citation Subset: IM |
Affiliation:
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Department of Andrology and Reproductive Endocrinology, Medical University of Lodz, Lodz, Poland. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Animals, Newborn Apoptosis / drug effects* Cell Differentiation / drug effects* Cell Proliferation / drug effects* Dose-Response Relationship, Drug Estradiol / pharmacology* Follicle Stimulating Hormone / pharmacology* Male Rats Rats, Wistar Seminiferous Tubules / cytology*, drug effects Sexual Maturation / drug effects, physiology Spermatogenesis / drug effects, physiology Spermatozoa / cytology, drug effects Testis / cytology, drug effects |
| Chemical | |
Reg. No./Substance:
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50-28-2/Estradiol; 9002-68-0/Follicle Stimulating Hormone |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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