Document Detail

Maturation-associated changes in the cellular composition of mouse calvariae and in the biochemical characteristics of calvarial cells separated into subclasses on Percoll density gradients.
MedLine Citation:
PMID:  1329439     Owner:  NLM     Status:  MEDLINE    
The effects of tissue maturation on the cellular composition and biochemical characteristics of bone were studied in neonatal, young adult, and aging mice. Osteoblast subclasses were isolated on Percoll density gradients. Neonatal calvariae consisted almost exclusively of cells banding at low and intermediate buoyant density. High buoyant density cells constituted 5-10% of total cells at 10 days of age but increased to 50-60% by 5 weeks of age. These latter cells were released late during collagenase digestion. This indicates that they arise from the deeper layer of bone. For this reason, we consider them putative osteocytes. We established that constitutive secretion of IGF-I and TGF-beta and activities of cellular alkaline phosphatase paralleled those of the tissue of origin in all cell groups and was highest in cells of intermediate buoyant density. These activities declined rapidly after cessation of growth at 5 weeks of age in both bone and isolated cells. Between 5 and 8 weeks of age, the hormonal response to PTH also declined dramatically. The maximum cAMP induced by PTH declined by about 70% in highly responsive cells of intermediate buoyant density and fell to insignificant levels in cells of high buoyant density. We found that a cyclic AMP response to PTH was positively correlated with stimulated secretion of IGF-I by this hormone in cells from animals of all ages. Despite their inability to respond to PTH with increases in cAMP and IGF-I, adult bone cells of high buoyant density continued to respond to PTH with increases in the secretion of TGF-beta.(ABSTRACT TRUNCATED AT 250 WORDS)
G L Wong; M C Ng
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research     Volume:  7     ISSN:  0884-0431     ISO Abbreviation:  J. Bone Miner. Res.     Publication Date:  1992 Jun 
Date Detail:
Created Date:  1992-11-10     Completed Date:  1992-11-10     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8610640     Medline TA:  J Bone Miner Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  701-8     Citation Subset:  IM    
University of Colorado, Colorado Springs.
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MeSH Terms
Age Factors
Cell Division
Cyclic AMP / physiology*
Insulin-Like Growth Factor I / drug effects,  secretion*
Models, Biological
Osteoblasts / cytology,  secretion*
Parathyroid Hormone / physiology*
Transforming Growth Factor beta / drug effects,  secretion*
Grant Support
Reg. No./Substance:
0/Parathyroid Hormone; 0/Transforming Growth Factor beta; 60-92-4/Cyclic AMP; 67763-96-6/Insulin-Like Growth Factor I

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