Document Detail

Maturation Competence of Swamp Buffalo Oocytes Obtained by Ovum Pick-Up and from Slaughterhouse Ovaries.
MedLine Citation:
PMID:  21749483     Owner:  NLM     Status:  Publisher    
This study was designed with the final goal of improving in vitro embryo production in the Thai swamp buffalo (Bubalus bubalis carabensis). Oocytes were collected by ovum pick-up (OPU) from six non-lactating multiparous swamp buffalo twice per week for 10 consecutive sessions followed by once-weekly collection for 10 consecutive sessions without hormone stimulation. In addition, oocytes were collected from slaughterhouse ovaries that were classified as follows: ovaries from non-pregnant cows with a visible corpus luteum (NPCL); pregnant cows with a corpus luteum (P); and non-pregnant cows without a corpus luteum (NP). Follicles in each group of ovaries were categorized as small (2-4 mm), medium-sized (5-8 mm) or large follicles (≥9 mm). The quality of the oocytes was assessed by their capacity to undergo in vitro maturation. The total number of observed follicles per session (all sizes combined) was larger in the once-weekly OPU group compared with the twice-weekly OPU group. In particular, the numbers of small and large follicles were higher in the once-weekly OPU group (5.2 ± 0.7 and 0.9 ± 0.2, respectively) than in the twice-weekly OPU group (3.9 ± 0.5 and 0.5 ± 0.1). The number of medium-sized follicles did not differ between the groups. The percentages of oocytes with an abnormal spindle morphology were not different between oocytes from the twice-weekly (30.0%) and the once-weekly (28.6%) OPU groups. A higher percentage of oocytes obtained in vitro (49.5%) exhibited nuclear abnormalities compared with those obtained in vivo (≤34.8%) after in vitro maturation. In conclusion, oocytes can be successfully collected by OPU in the swamp buffalo, without hormonal pretreatment, and per week more good-quality oocytes can be collected by twice-weekly OPU. In addition, oocytes collected from slaughterhouse ovaries can be used with the reproductive status of the cow having no influence on the maturation competence of oocytes.
M Yindee; M Techakumphu; C Lohachit; S Sirivaidyapong; A Na-Chiangmai; Baj Roelen; B Colenbrander
Related Documents :
21741783 - Induction/synchronization of oestrus and ovulation in dairy goats with different short ...
16416613 - Are patients better than the laboratory in assessing sweating? validation study.
1853913 - Ten years after cryosurgical treatment of cervical intraepithelial neoplasia.
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-7-12
Journal Detail:
Title:  Reproduction in domestic animals = Zuchthygiene     Volume:  -     ISSN:  1439-0531     ISO Abbreviation:  -     Publication Date:  2011 Jul 
Date Detail:
Created Date:  2011-7-13     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9015668     Medline TA:  Reprod Domest Anim     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
© 2011 Blackwell Verlag GmbH.
Faculty of Veterinary Science, Department of Obstetrics Gynecology and Reproduction, Chulalongkorn University, Bangkok, Thailand Faculty of Veterinary Medicine, Department of Farm Animal Health, Utrecht University, Utrecht, The Netherlands Buffalo Research and Development Group, Department of Livestock Development, Bangkok, Thailand.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Oral health promotion interventions on oral yeast in hospitalised and medically compromised patients...
Next Document:  Electrocardiographic Changes in Normal and Abnormal Canine Pregnancy.