Document Detail


Matrix synthesis in high density cultures of bovine epiphyseal plate chondrocytes.
MedLine Citation:
PMID:  6221876     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Bovine epiphyseal plate chondrocytes were cultured by a method combining both suspension culture and high density monolayers. The matrix synthesized by the cultured cells was analyzed at fifteen days for glycosaminoglycan, proteoglycan, and collagen content. In the cell culture product glycosaminoglycan distribution was: 65% chondroitin-6-sulfate, 18% chondroitin-4-sulfate, 15% keratan sulfate, and less than 2% dermatan sulfate. Essentially all the radioactive sulfate in labelled specimens was present in high molecular weight aggregates. The collagen which was synthesized co-migrated with Type II collagen standard. Parallel analysis showed the matrix of cultured cells to be similar to that of intact epiphyseal plate tissue. This study demonstrates the ability to grow epiphyseal plate chondrocytes in a cell culture system which allows matrix synthesis similar to that seen in vivo.
Authors:
P C Amadio; M G Ehrlich; H J Mankin
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Connective tissue research     Volume:  11     ISSN:  0300-8207     ISO Abbreviation:  Connect. Tissue Res.     Publication Date:  1983  
Date Detail:
Created Date:  1983-07-08     Completed Date:  1983-07-08     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0365263     Medline TA:  Connect Tissue Res     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  11-9     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Cartilage / cytology,  metabolism*
Cattle
Cells, Cultured
Chromatography, Thin Layer
Collagen / biosynthesis*
Electrophoresis, Polyacrylamide Gel
Epiphyses
Glycosaminoglycans / biosynthesis*
Histocytochemistry
Proteoglycans / biosynthesis*
Grant Support
ID/Acronym/Agency:
R01 AM 16265-10/AM/NIADDK NIH HHS
Chemical
Reg. No./Substance:
0/Glycosaminoglycans; 0/Proteoglycans; 9007-34-5/Collagen

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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