| Matrix synthesis in high density cultures of bovine epiphyseal plate chondrocytes. | |
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MedLine Citation:
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PMID: 6221876 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Bovine epiphyseal plate chondrocytes were cultured by a method combining both suspension culture and high density monolayers. The matrix synthesized by the cultured cells was analyzed at fifteen days for glycosaminoglycan, proteoglycan, and collagen content. In the cell culture product glycosaminoglycan distribution was: 65% chondroitin-6-sulfate, 18% chondroitin-4-sulfate, 15% keratan sulfate, and less than 2% dermatan sulfate. Essentially all the radioactive sulfate in labelled specimens was present in high molecular weight aggregates. The collagen which was synthesized co-migrated with Type II collagen standard. Parallel analysis showed the matrix of cultured cells to be similar to that of intact epiphyseal plate tissue. This study demonstrates the ability to grow epiphyseal plate chondrocytes in a cell culture system which allows matrix synthesis similar to that seen in vivo. |
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Authors:
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P C Amadio; M G Ehrlich; H J Mankin |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Connective tissue research Volume: 11 ISSN: 0300-8207 ISO Abbreviation: Connect. Tissue Res. Publication Date: 1983 |
Date Detail:
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Created Date: 1983-07-08 Completed Date: 1983-07-08 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0365263 Medline TA: Connect Tissue Res Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 11-9 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cartilage / cytology, metabolism* Cattle Cells, Cultured Chromatography, Thin Layer Collagen / biosynthesis* Electrophoresis, Polyacrylamide Gel Epiphyses Glycosaminoglycans / biosynthesis* Histocytochemistry Proteoglycans / biosynthesis* |
| Grant Support | |
ID/Acronym/Agency:
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R01 AM 16265-10/AM/NIADDK NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Glycosaminoglycans; 0/Proteoglycans; 9007-34-5/Collagen |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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