| Matrix simulation of duodenal crypt cell kinetics. II. Cell kinetics following hydroxyurea. | |
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MedLine Citation:
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PMID: 1170948 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The perturbed cellular kinetics of the duodenal crypt following a single injection of hydroxyurea (HU) have been simulated using matrix algebra. Following the direct effects of HU (S-phase cytotoxicity and a G1/S block) the crypt cell kinetics undergo several alterations. Previously documented alterations include: (1) a temporary partial synchronization of the surviving cells, (2) a shortening of the cell-cycle transit time, and (3) recruitment of normally non-proliferating cells into active proliferation. These conclusions have been extended by constructing several different complex but theoretically possible recovery models and the validity of each of these models has been evaluated by simulating the following biological data: the number of cells in the S and M-phase of the cell cycle, total viable cells per crypt, and the per cent labeled mitosis and the number of labeled cells following 3H-TdR injections at 9 and 21 hr after HU treatment. The model which showed visually the best overall agreement with all sets of the data was chosen as "most probable' and leads to the following interpretations. Immediately after the end of the HU block (i.e. 5 hr after HU injection) the modal cell-cycle transit time is reduced to 8 hr. By 17 hr after HU, the modal transit time is increased to 10 hr. Repopulation of the proliferating compartment, i.e. restoration of the proliferating compartment back to the control value, occurs between 12 and 17 hr after HU injection and probably consists of both recycling of the proliferating cells (i.e. they do not progress up into the non-proliferating compartment) and recruitment of the non-proliferating cells into active proliferation. Also, the rate at which the non-proliferating cells move onto the villi is reduced temporarily. The overall recovery process results in a crypt which temporarily is larger than control and produces villi cells at a rate which is faster than the control. The time when the crypt size and villus cell production rate return to normal cannot be established using the available data. |
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Authors:
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J L Roti Roti; L A Dethlefsen |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Cell and tissue kinetics Volume: 8 ISSN: 0008-8730 ISO Abbreviation: Cell Tissue Kinet Publication Date: 1975 Jul |
Date Detail:
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Created Date: 1975-11-06 Completed Date: 1975-11-06 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0174107 Medline TA: Cell Tissue Kinet Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 335-53 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Count Cell Division / drug effects* Cell Survival Cells, Cultured Duodenum / cytology Hydroxyurea / pharmacology* Intestinal Mucosa / cytology Isotope Labeling Kinetics Mathematics Mice Mice, Inbred C3H Mitosis / drug effects Models, Biological* Thymidine Tritium |
| Chemical | |
Reg. No./Substance:
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10028-17-8/Tritium; 127-07-1/Hydroxyurea; 50-89-5/Thymidine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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