| Matrix metalloproteinases and tissue inhibitors of metalloproteinases in patients with different types of scars and keloids. | |
| | |
MedLine Citation:
|
PMID: 19464975 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
BACKGROUND: Hypertrophic scars and keloids are fibroproliferative skin disorders characterised by progressive deposition of collagen. Our study is designed to investigate the expression and concentration of matrix metalloproteinases (MMPs) and tissue inhibitors of metalloproteinases (TIMPs) in different types of scars and keloids. METHODS: Total RNA from 19 proliferative hypertrophic scar samples of patients with extended burns (total body surface area (TBSA): 21+/-12%), 18 mature hypertrophic scar samples from patients after elective surgery, 14 keloid samples and 18 normotrophic scar samples was, respectively, extracted, and then mRNA was isolated. Besides, biopsies were obtained from non-scarred skin of the patients and extraction of total RNA performed. Relative mRNA expression of MMP 2, MMP 9, TIMP 1 and TIMP 2 was measured with reverse transcriptase polymerase chain reaction (RT-PCR). Serum concentrations of MMP-1, -2, -9, TIMP-1, and -2 were determined using an enzyme-linked immunosorbent assay (ELISA). RESULTS: Patients with extended hypertrophic scars after burn trauma presented a significantly higher TIMP-1 concentration (p<0.05) in their sera than the other patients. The relative expression of MMP 2 was significantly higher in samples of proliferative hypertrophic scars after burn injury. The relative expression of TIMP 1 and TIMP 2 was significantly higher in scar tissue of patients with proliferative and mature hypertrophic scars and keloids than in their regular skin and in scar samples of patients with normotrophic scars. The expression of TIMP 1 was significantly higher in samples of patients with keloids than in patients with hypertrophic scars. CONCLUSIONS: The concentration of TIMP-1 in sera of patients varies depending on the size of the involved fibrotic scar tissue. A decrease in MMP-to-TIMP expression in scar tissue may contribute to increased synthesis and deposition of collagen, leading to a severe fibrotic reaction with pathologic scar formation. The results implicate non-operative therapy options in these patients that not only down-regulate TIMPs but also increase the activity of MMPs. |
| | |
Authors:
|
Dietmar Ulrich; Franziska Ulrich; Frank Unglaub; Andrzej Piatkowski; Norbert Pallua |
Related Documents
:
|
3126305 - Factors influencing energy expenditure in patients with burns. 21815705 - Atorvastatin metabolite measurements as a diagnostic tool for statin-induced myopathy. 18552695 - Lipopolysaccharide binding protein in a surgical intensive care unit: a marker of sepsis? 6688005 - Atracurium in the severely ill. 8288665 - Cuneiform osteotomy of the femoral neck in the treatment of slipped capital femoral epi... 6149715 - Clinical and prognostic significance of vasculitis as an early manifestation of connect... |
Publication Detail:
|
Type: Journal Article Date: 2009-05-22 |
Journal Detail:
|
Title: Journal of plastic, reconstructive & aesthetic surgery : JPRAS Volume: 63 ISSN: 1878-0539 ISO Abbreviation: J Plast Reconstr Aesthet Surg Publication Date: 2010 Jun |
Date Detail:
|
Created Date: 2010-05-10 Completed Date: 2010-06-10 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 101264239 Medline TA: J Plast Reconstr Aesthet Surg Country: Netherlands |
Other Details:
|
Languages: eng Pagination: 1015-21 Citation Subset: IM |
Copyright Information:
|
Copyright 2009 British Association of Plastic, Reconstructive and Aesthetic Surgeons. Published by Elsevier Ltd. All rights reserved. |
Affiliation:
|
Department of Plastic Surgery, Hand Surgery, Burn Unit, University Hospital Aachen, Medical Faculty of the University of Technology, Pauwelsstrasse 30, 52074 Aachen, Germany. dulrich@ukaachen.de |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Adolescent Adult Burns / blood, enzymology*, pathology Case-Control Studies Cicatrix, Hypertrophic / blood, enzymology*, etiology Cohort Studies Female Humans Keloid / blood, enzymology*, etiology Male Matrix Metalloproteinases, Secreted / genetics, metabolism* Middle Aged RNA, Messenger / metabolism Reverse Transcriptase Polymerase Chain Reaction Tissue Inhibitor of Metalloproteinases / genetics, metabolism* Young Adult |
| Chemical | |
Reg. No./Substance:
|
0/RNA, Messenger; 0/Tissue Inhibitor of Metalloproteinases; EC 3.4.24.-/Matrix Metalloproteinases, Secreted |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Adaptation of the Hall-Findlay technique for simultaneous contralateral reduction in delayed breast ...
Next Document: Informed choice and diabetes screening in primary care: qualitative study of patient and professiona...