Document Detail

Matrix metalloproteinases and tissue inhibitors of metalloproteinases in patients with different types of scars and keloids.
MedLine Citation:
PMID:  19464975     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: Hypertrophic scars and keloids are fibroproliferative skin disorders characterised by progressive deposition of collagen. Our study is designed to investigate the expression and concentration of matrix metalloproteinases (MMPs) and tissue inhibitors of metalloproteinases (TIMPs) in different types of scars and keloids. METHODS: Total RNA from 19 proliferative hypertrophic scar samples of patients with extended burns (total body surface area (TBSA): 21+/-12%), 18 mature hypertrophic scar samples from patients after elective surgery, 14 keloid samples and 18 normotrophic scar samples was, respectively, extracted, and then mRNA was isolated. Besides, biopsies were obtained from non-scarred skin of the patients and extraction of total RNA performed. Relative mRNA expression of MMP 2, MMP 9, TIMP 1 and TIMP 2 was measured with reverse transcriptase polymerase chain reaction (RT-PCR). Serum concentrations of MMP-1, -2, -9, TIMP-1, and -2 were determined using an enzyme-linked immunosorbent assay (ELISA). RESULTS: Patients with extended hypertrophic scars after burn trauma presented a significantly higher TIMP-1 concentration (p<0.05) in their sera than the other patients. The relative expression of MMP 2 was significantly higher in samples of proliferative hypertrophic scars after burn injury. The relative expression of TIMP 1 and TIMP 2 was significantly higher in scar tissue of patients with proliferative and mature hypertrophic scars and keloids than in their regular skin and in scar samples of patients with normotrophic scars. The expression of TIMP 1 was significantly higher in samples of patients with keloids than in patients with hypertrophic scars. CONCLUSIONS: The concentration of TIMP-1 in sera of patients varies depending on the size of the involved fibrotic scar tissue. A decrease in MMP-to-TIMP expression in scar tissue may contribute to increased synthesis and deposition of collagen, leading to a severe fibrotic reaction with pathologic scar formation. The results implicate non-operative therapy options in these patients that not only down-regulate TIMPs but also increase the activity of MMPs.
Dietmar Ulrich; Franziska Ulrich; Frank Unglaub; Andrzej Piatkowski; Norbert Pallua
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Publication Detail:
Type:  Journal Article     Date:  2009-05-22
Journal Detail:
Title:  Journal of plastic, reconstructive & aesthetic surgery : JPRAS     Volume:  63     ISSN:  1878-0539     ISO Abbreviation:  J Plast Reconstr Aesthet Surg     Publication Date:  2010 Jun 
Date Detail:
Created Date:  2010-05-10     Completed Date:  2010-06-10     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101264239     Medline TA:  J Plast Reconstr Aesthet Surg     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  1015-21     Citation Subset:  IM    
Copyright Information:
Copyright 2009 British Association of Plastic, Reconstructive and Aesthetic Surgeons. Published by Elsevier Ltd. All rights reserved.
Department of Plastic Surgery, Hand Surgery, Burn Unit, University Hospital Aachen, Medical Faculty of the University of Technology, Pauwelsstrasse 30, 52074 Aachen, Germany.
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MeSH Terms
Burns / blood,  enzymology*,  pathology
Case-Control Studies
Cicatrix, Hypertrophic / blood,  enzymology*,  etiology
Cohort Studies
Keloid / blood,  enzymology*,  etiology
Matrix Metalloproteinases, Secreted / genetics,  metabolism*
Middle Aged
RNA, Messenger / metabolism
Reverse Transcriptase Polymerase Chain Reaction
Tissue Inhibitor of Metalloproteinases / genetics,  metabolism*
Young Adult
Reg. No./Substance:
0/RNA, Messenger; 0/Tissue Inhibitor of Metalloproteinases; EC 3.4.24.-/Matrix Metalloproteinases, Secreted

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