| Matrix metalloproteinases, MMP-7 and MMP-26, in plasma and serum of control and preeclamptic umbilical cord blood. | |
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MedLine Citation:
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PMID: 20371146 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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OBJECTIVE: Our previous paper demonstrated that preeclampsia-associated accumulation of collagen and proteoglycans in the umbilical cord tissues is a result of increased biosynthesis and decreased degradation of these components. Metalloproteinases (MMPs) are enzymes engaged in degradation of collagen and protein cores of proteoglycans, including those which bind peptide growth factors. Some MMPs, among them matrilysins MMP-7 and MMP-26, participate in activation other members of the MMP family. STUDY DESIGN: Studies were performed on the umbilical cord blood taken from 10 control (healthy) newborns and 10 newborns of preeclamptic women. We used Western immunoblotting, immunoenzymatic assay (ELISA) and zymography techniques for detection of matrilysins. The results were submitted to Student's t-test and Mann-Whitney test. RESULTS: Umbilical cord blood plasma and serum of control and preeclamptic newborns contained MMP-7 and MMP-26. Both enzymes existed in the form of complexes with other extracellular matrix components and/or their tissue inhibitors in control and preeclamptic subjects. Free latent forms of both matrilysins were observed after the action of reducing agent. Furthermore, we found a distinct increase in the amount of MMP-26 in preeclamptic umbilical cord (UC) blood. No significant differences in MMP-7 content and activity in control and preeclamptic UC blood were observed. CONCLUSIONS: MMP-7 and MMP-26 could activate MMP-9 by cleavage of some sites in pro-MMP-9. Our results suggest that the high activity of MMP-9 participates in a proteolytic release of peptide growth factors from their complexes with extracellular matrix components, which facilitate their interaction with membrane receptors and stimulate cell division and extracellular matrix synthesis in these cells. It may be one of the mechanisms of extracellular matrix remodelling in the umbilical cord of preeclamptic newborns. |
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Authors:
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Zofia Galewska; Lech Romanowicz; Stefan Jaworski; Edward Bańkowski |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-04-03 |
Journal Detail:
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Title: European journal of obstetrics, gynecology, and reproductive biology Volume: 150 ISSN: 1872-7654 ISO Abbreviation: Eur. J. Obstet. Gynecol. Reprod. Biol. Publication Date: 2010 Jun |
Date Detail:
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Created Date: 2010-05-17 Completed Date: 2010-08-13 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0375672 Medline TA: Eur J Obstet Gynecol Reprod Biol Country: Ireland |
Other Details:
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Languages: eng Pagination: 152-6 Citation Subset: IM |
Copyright Information:
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Copyright 2010 Elsevier Ireland Ltd. All rights reserved. |
Affiliation:
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Department of Medical Biochemistry, Medical University of Białystok, Białystok, Poland. zofia.galewska@umwb.edu.pl |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adult Blotting, Western Enzyme-Linked Immunosorbent Assay Female Fetal Blood Humans Infant, Newborn Matrix Metalloproteinase 7 / blood* Matrix Metalloproteinases, Secreted / blood* Pre-Eclampsia / blood* Pregnancy Statistics, Nonparametric |
| Chemical | |
Reg. No./Substance:
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EC 3.4.24.-/MMP26 protein, human; EC 3.4.24.-/Matrix Metalloproteinases, Secreted; EC 3.4.24.23/Matrix Metalloproteinase 7 |
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