Document Detail

Matrix metalloproteinases MMP-2, -9 and tissue inhibitors TIMP-1, -2 expression and secretion by primary human osteoblast cells in response to titanium, zirconia, and alumina ceramics.
MedLine Citation:
PMID:  14661256     Owner:  NLM     Status:  MEDLINE    
Osteogenic properties of bone cells are a key parameter governing osseointegration of implant devices. In this context, osteoblasts have a central role via extracellular matrix synthesis and remodeling that they regulate through different protease activity. In this study, we have analyzed the expression of two matrix metalloproteinases (MMPs): MMP-2 (72 kDa) and MMP-9 (92 kDa) and their specific tissue inhibitors TIMP-1 and TIMP-2 in primary human osteoblastic cells. The effect of titanium, zirconia, and alumina ceramics on the synthesis of these proteases was assessed using reverse transcriptase-polymerase chain reaction, enzyme-linked immunosorbent assay, and zymographic analysis. Our results showed that osteoblasts express MMP-2 and -9 mRNA. Furthermore, MMP-2 mRNA expression was decreased by titanium and increased by alumina whereas zirconia did not have any significant effect. Conversely, MMP-9 mRNA expression was stimulated by titanium but decreased with zirconia, whereas alumina induced no significant changes. Zymographic analysis has evidenced pro-MMP-2 gelatinolytic activity in all cell populations with time-dependent increase profile; pro-MMP-9, however, was not detected. Enzyme-linked immunosorbent assay data confirmed the production of MMP-2 and very low levels of MMP-9. In addition, TIMP-1 was secreted in 24-h-cultured cells and increased to maximal level at 48-72 h whereas TIMP-2 levels were very low. The interactions between human osteoblasts and the studied biomaterials altered both MMP-2, -9 and TIMP-1expression indicating that biomaterials may influence osseointegration and bone remodeling.
Z Oum'hamed; R Garnotel; Y Josset; C Trenteseaux; D Laurent-Maquin
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of biomedical materials research. Part A     Volume:  68     ISSN:  1549-3296     ISO Abbreviation:  -     Publication Date:  2004 Jan 
Date Detail:
Created Date:  2003-12-08     Completed Date:  2004-08-30     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  101234237     Medline TA:  J Biomed Mater Res A     Country:  United States    
Other Details:
Languages:  eng     Pagination:  114-22     Citation Subset:  IM    
Copyright Information:
Copyright 2003 Wiley Periodicals, Inc. J Biomed Mater Res 68A: 114-122, 2004
Unité INSERM ERM 0203, IFR 53, UFR Odontologie-1, Faculté de Pharmacie/Médecine 1, Ave du Maréchal Juin, 51095 Reims Cedex, France.
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MeSH Terms
Aluminum Oxide / pharmacology*
Base Sequence
Ceramics / pharmacology*
DNA Primers
Enzyme-Linked Immunosorbent Assay
Matrix Metalloproteinase 2 / genetics*,  secretion
Matrix Metalloproteinase 9 / genetics*,  secretion
Osteoblasts / drug effects,  enzymology,  metabolism*
Reverse Transcriptase Polymerase Chain Reaction
Tissue Inhibitor of Metalloproteinase-1 / genetics*,  secretion
Tissue Inhibitor of Metalloproteinase-2 / genetics*,  secretion
Titanium / pharmacology*
Zirconium / pharmacology*
Reg. No./Substance:
0/Ceramics; 0/DNA Primers; 0/Tissue Inhibitor of Metalloproteinase-1; 127497-59-0/Tissue Inhibitor of Metalloproteinase-2; 1314-23-4/zirconium oxide; 1344-28-1/Aluminum Oxide; 7440-32-6/Titanium; 7440-67-7/Zirconium; EC Metalloproteinase 2; EC Metalloproteinase 9

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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