| Matrix metalloproteinases, IL-6, and nitric oxide in rat antigen-induced arthritis. | |
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MedLine Citation:
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PMID: 9631748 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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OBJECTIVE: Degradation of extracellular matrix by matrix metalloproteinases (MMPs) is believed to be important in processes leading to the progressive destruction of joints in rheumatoid arthritis (RA). We used the model of antigen-induced arthritis (AIA) to study MMP activity and the influence of cytokines on their expression by synoviocytes. MATERIAL AND METHODS: Procedures used were: mBSA-induced arthritis in rats; investigation of synovial fluids and supernatants of cultured synoviocytes at different time points during AIA; estimation of total MMP activities by fluorescence assay; zymographic investigations; IL-6 bioassay with B9 cells; nitric oxide (NO) estimation. RESULTS: Total MMP activity in the synovial fluids of arthritic joints was higher than in the contralateral joints or in the joints of untreated control animals. The maximum was noted between day 7 and day 14 after arthritis induction. Cultured synoviocytes, prepared at different time points after arthritis induction, secreted MMPs into the media with a maximum time lapse of 14 days after arthritis induction. TNF-alpha increased the total MMP activity released. Moreover, TNF-alpha as well as IL-1 beta induced the expression of MMP9. Finally, TNF-alpha increased the levels of IL-6 and NO in the supernatants of synoviocytes; the extent of stimulation was dependent on the course of AIA. CONCLUSIONS: Different MMPs are synthesized in varying concentrations during the course of rat AIA. Cytokines such as TNF-alpha and IL-1 beta differentially influence the activity and expression of MMPs in cultured synoviocytes. The participation of MMPs in tissue degradation during the course of arthritis may be of importance for the development of new therapeutic strategies. |
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Authors:
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K Mentzel; R Bräuer |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Clinical and experimental rheumatology Volume: 16 ISSN: 0392-856X ISO Abbreviation: Clin. Exp. Rheumatol. Publication Date: 1998 May-Jun |
Date Detail:
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Created Date: 1998-08-24 Completed Date: 1998-08-24 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8308521 Medline TA: Clin Exp Rheumatol Country: ITALY |
Other Details:
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Languages: eng Pagination: 269-76 Citation Subset: IM |
Affiliation:
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Institute of Pathology, Friedrich Schiller University Jena, Germany. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Arthritis, Reactive / enzymology*, immunology* Cells, Cultured Enzyme Activation / drug effects Female Gelatin / metabolism Interleukin-1 / pharmacology Interleukin-6 / analysis, immunology, metabolism* Male Metalloendopeptidases / metabolism* Nitric Oxide / metabolism* Rats Rats, Wistar Synovial Fluid / chemistry, enzymology Synovial Membrane / chemistry, cytology, enzymology Tumor Necrosis Factor-alpha / pharmacology |
| Chemical | |
Reg. No./Substance:
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0/Interleukin-1; 0/Interleukin-6; 0/Tumor Necrosis Factor-alpha; 10102-43-9/Nitric Oxide; 9000-70-8/Gelatin; EC 3.4.24.-/Metalloendopeptidases |
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