Document Detail

Matrix metalloproteinase distribution during early corneal wound healing.
MedLine Citation:
PMID:  15332107     Owner:  NLM     Status:  MEDLINE    
AIM: To compare matrix metalloproteinase (MMP) localisation in anterior keratectomy (AK) and lamellar keratectomy (LK) wounds. METHODS: Wounds were produced in one eye of 24 rabbits. The AK wounds were made to approximately 120 microm in depth and then allowed to re-epithelialise. The LK wounds were of similar depth, but the anterior stroma and epithelium were replaced after a second deeper keratectomy had been performed. Immunohistochemistry was used to localise the MMP-1, -2, -3, and -9 at intervals from 4 h to 14 days following surgery. The contralateral eyes acted as controls. RESULTS: After an AK wound MMP-1 was present at the leading edge of migrating epithelium after 18 h, while MMP-2 and -9 were localised behind the advancing epithelial edge. The presence of these enzymes rapidly fell to low levels after epithelial closure. There was only faint MMP-3 localisation between days 3 and 7. After an LK wound, MMP-1, -3, and -9 were not detected in the stromal interface, but MMP-2 was present at all time points. CONCLUSIONS: This study suggests that after an AK wound, MMP-1 is a key mediator of epithelial migration, while MMP-2 and -9, and to a lesser extent MMP-3, may participate in the remodelling of corneal stroma and the reformation of epithelial basement membrane. In contrast, an LK wound results in a much lower stimulus for MMP activation. The action of MMP-2 in stromal repair is thus partly independent of epithelial injury.
B Mulholland; S J Tuft; P T Khaw
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Eye (London, England)     Volume:  19     ISSN:  0950-222X     ISO Abbreviation:  Eye (Lond)     Publication Date:  2005 May 
Date Detail:
Created Date:  2005-05-04     Completed Date:  2005-08-23     Revised Date:  2009-11-03    
Medline Journal Info:
Nlm Unique ID:  8703986     Medline TA:  Eye (Lond)     Country:  England    
Other Details:
Languages:  eng     Pagination:  584-8     Citation Subset:  IM    
Wound Healing Research Unit, Division of Pathology, Institute of Ophthalmology, London, UK.
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MeSH Terms
Cornea / enzymology*,  physiology
Corneal Stroma / enzymology
Corneal Surgery, Laser*
Epithelium, Corneal / enzymology
Keratomileusis, Laser In Situ
Lasers, Excimer
Matrix Metalloproteinase 1 / metabolism
Matrix Metalloproteinase 2 / metabolism
Matrix Metalloproteinase 3 / metabolism
Matrix Metalloproteinase 9 / metabolism
Matrix Metalloproteinases / metabolism*,  physiology
Photorefractive Keratectomy
Postoperative Period
Wound Healing*
Reg. No./Substance:
EC 3.4.24.-/Matrix Metalloproteinases; EC Metalloproteinase 3; EC Metalloproteinase 2; EC Metalloproteinase 9; EC Metalloproteinase 1

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