Document Detail

Matrix metalloproteinase 7 and perlecan in oral epithelial dysplasia and carcinoma in situ: an aid for histopathologic recognition of their cell proliferation centers.
MedLine Citation:
PMID:  19239574     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: As one of the valuable tools for differential diagnoses of oral epithelial dysplasia, carcinoma in situ (CIS) and squamous cell carcinoma (SCC), we have proposed the immunohistochemistry for perlecan, a heparan sulfate proteoglycan (HSPG). As HSPGs have been shown to be extracellular docking molecules for matrix metalloproteinase (MMP) 7, our aim was to determine the expression mode of MMP-7 in these lesions for its possible diagnostic aid for oral borderline malignancies. METHODS: Twenty cases each of moderate dysplasia, CIS, SCC, and normal/hyperplastic/mild dysplastic epithelia of the tongue and buccal mucosa were immunohistochemically examined for MMP-1, -2 and -7 in reference to their perlecan immunolocalization. RESULTS: The expression of all three MMPs in the normal mucosal epithelium was restricted mainly to the parabasal layers. The most striking finding was strong expression of MMP-7 in epithelial dysplasia with a two-phase appearance: a clear demarcation of MMP-7-immunopositive (+) lower dysplastic/basaloid cells from non-positive upper keratinized cells. MMP-7+ cells were spread over the whole epithelial layer of CIS. In SCC, MMP-7 positivity was reduced from carcinoma cells but instead appeared in stromal cells. These expression profiles of MMP-7 resembled those of perlecan. MMP-1 and MMP-2 exhibited a similar but much weaker staining than MMP-7. CONCLUSION: These results suggest that the enhanced metabolism of perlecan associated with MMP-7 plays an important role in the cell proliferation of oral epithelia in their malignant transformation process, and that MMP-7 immunohistochemistry may be a valuable aid for identification of the cell proliferation center in oral CIS and dysplasia.
W M Tilakaratne; T Kobayashi; H Ida-Yonemochi; W Swelam; M Yamazaki; T Mikami; C G Alvarado; A Md Shahidul; S Maruyama; J Cheng; T Saku
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-02-23
Journal Detail:
Title:  Journal of oral pathology & medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology     Volume:  38     ISSN:  1600-0714     ISO Abbreviation:  J. Oral Pathol. Med.     Publication Date:  2009 Apr 
Date Detail:
Created Date:  2009-04-01     Completed Date:  2009-07-07     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8911934     Medline TA:  J Oral Pathol Med     Country:  Denmark    
Other Details:
Languages:  eng     Pagination:  348-55     Citation Subset:  D; IM    
Division of Oral Pathology, Niigata University Graduate School of Medical and Dental Sciences, Niigata, Japan.
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MeSH Terms
Carcinoma in Situ / diagnosis,  metabolism*
Carcinoma, Squamous Cell / diagnosis,  metabolism*
Cell Proliferation
Cell Transformation, Neoplastic / metabolism*
Diagnosis, Differential
Epithelial Cells / metabolism,  pathology
Heparan Sulfate Proteoglycans / metabolism*
Matrix Metalloproteinase 1 / biosynthesis
Matrix Metalloproteinase 2 / biosynthesis
Matrix Metalloproteinase 7 / biosynthesis*
Precancerous Conditions / diagnosis,  metabolism*
Tongue Neoplasms / diagnosis,  metabolism*
Reg. No./Substance:
0/Heparan Sulfate Proteoglycans; 143972-95-6/perlecan; EC Metalloproteinase 7; EC Metalloproteinase 2; EC Metalloproteinase 1

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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