| Matrix-insensitive protein assays push the limits of biosensors in medicine. | |
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MedLine Citation:
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PMID: 19820717 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Advances in biosensor technologies for in vitro diagnostics have the potential to transform the practice of medicine. Despite considerable work in the biosensor field, there is still no general sensing platform that can be ubiquitously applied to detect the constellation of biomolecules in diverse clinical samples (for example, serum, urine, cell lysates or saliva) with high sensitivity and large linear dynamic range. A major limitation confounding other technologies is signal distortion that occurs in various matrices due to heterogeneity in ionic strength, pH, temperature and autofluorescence. Here we present a magnetic nanosensor technology that is matrix insensitive yet still capable of rapid, multiplex protein detection with resolution down to attomolar concentrations and extensive linear dynamic range. The matrix insensitivity of our platform to various media demonstrates that our magnetic nanosensor technology can be directly applied to a variety of settings such as molecular biology, clinical diagnostics and biodefense. |
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Authors:
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Richard S Gaster; Drew A Hall; Carsten H Nielsen; Sebastian J Osterfeld; Heng Yu; Kathleen E Mach; Robert J Wilson; Boris Murmann; Joseph C Liao; Sanjiv S Gambhir; Shan X Wang |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Technical Report Date: 2009-10-11 |
Journal Detail:
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Title: Nature medicine Volume: 15 ISSN: 1546-170X ISO Abbreviation: Nat. Med. Publication Date: 2009 Nov |
Date Detail:
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Created Date: 2009-11-06 Completed Date: 2009-11-23 Revised Date: 2010-04-29 |
Medline Journal Info:
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Nlm Unique ID: 9502015 Medline TA: Nat Med Country: United States |
Other Details:
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Languages: eng Pagination: 1327-32 Citation Subset: IM |
Affiliation:
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Department of Bioengineering, Stanford University, Stanford, California, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Biological Assay* Biosensing Techniques / instrumentation*, methods Carcinoembryonic Antigen / metabolism Disease Models, Animal Enzyme-Linked Immunosorbent Assay / methods Humans Hydrogen-Ion Concentration Magnetic Resonance Spectroscopy / methods Mice Nanotechnology / instrumentation, methods Optics and Photonics / instrumentation Proteins / metabolism* Reproducibility of Results Sensitivity and Specificity Temperature Time Factors Vascular Endothelial Growth Factor A / metabolism Xenograft Model Antitumor Assays / instrumentation, methods |
| Grant Support | |
ID/Acronym/Agency:
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1U54CA119367/CA/NCI NIH HHS; N44CM-2009-00011/CM/NCI NIH HHS; P50 CA114747/CA/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Carcinoembryonic Antigen; 0/Proteins; 0/Vascular Endothelial Growth Factor A |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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