| Matrix effects in the determination of zinc(II) ion in whole rat liver by flame atomic absorption spectrometry. | |
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MedLine Citation:
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PMID: 7741256 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Analytical systems that might be of practical importance for the direct determination of ZnII ion in whole rat liver homogenates by flame atomic absorption spectrometry were reliably evaluated based on the change in calibration sensitivity. For that purpose synthetic samples were prepared in three extremely different media for tissue homogenization. Hydrochloric acid medium proved to be more resistant to matrix components (non-ionic detergent, albumin, inorganic salts) than water or Tris-acetate buffer (pH 8.7). Further, a highly acidic medium allows the substitution of complex albumin and salt-containing standard solution with simple acidic and even aqueous solutions. The convenience of 1.0 mol l-1 HCl as the medium for rat liver homogenization is emphasized, owing not only to quantitative extraction of metal ions but also to markedly reduced matrix effects. |
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Authors:
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S Luterotti |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: The Analyst Volume: 120 ISSN: 0003-2654 ISO Abbreviation: Analyst Publication Date: 1995 Mar |
Date Detail:
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Created Date: 1995-06-02 Completed Date: 1995-06-02 Revised Date: 2003-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0372652 Medline TA: Analyst Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 925-30 Citation Subset: IM |
Affiliation:
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Department of Analytical Chemistry, Faculty of Pharmacy and Biochemistry, University of Zagreb, Croatia. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Acetates Animals Buffers Hydrochloric Acid Hydrogen-Ion Concentration Liver / chemistry* Rats Sensitivity and Specificity Spectrophotometry, Atomic / statistics & numerical data* Tromethamine Water Zinc / analysis* |
| Chemical | |
Reg. No./Substance:
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0/Acetates; 0/Buffers; 7440-66-6/Zinc; 7647-01-0/Hydrochloric Acid; 77-86-1/Tromethamine; 7732-18-5/Water |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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