| Matrix-assisted laser desorption/ionisation time-of-flight (MALDI-TOF) mass spectrometry as a tool for rapid diagnosis of potentially toxigenic Corynebacterium species in the laboratory management of diphtheria-associated bacteria. | |
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MedLine Citation:
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PMID: 21087580 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The rapid identification of the potentially toxigenic Corynebacterium species, C. diphtheriae, C. ulcerans and C. pseudotuberculosis is essential for diagnosis and treatment of diphtheria and diphtheria-like diseases. We used matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MALDIT-OF MS) in comparison with classical microbiological and molecular methods on 116 Corynebacterium strains. All 90 potentially toxigenic Corynebacterium strains collected by the German National Consiliary Laboratory on Diphtheria in a period of more than ten years were correctly identified by MALDI-TOF MS. We propose an algorithm for fast and reliable diagnosis of diphtheria incorporating MALDI-TOF MS, real-time tox PCR and Elek testing. |
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Authors:
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R Konrad; A Berger; I Huber; V Boschert; S Hörmansdorfer; U Busch; M Hogardt; S Schubert; A Sing |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-10-28 |
Journal Detail:
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Title: Euro surveillance : bulletin européen sur les maladies transmissibles = European communicable disease bulletin Volume: 15 ISSN: 1560-7917 ISO Abbreviation: Euro Surveill. Publication Date: 2010 |
Date Detail:
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Created Date: 2010-11-22 Completed Date: 2011-01-20 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 100887452 Medline TA: Euro Surveill Country: Sweden |
Other Details:
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Languages: eng Pagination: - Citation Subset: IM |
Affiliation:
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National Consiliary Laboratory for Diphtheria, Oberschleissheim, Germany. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Algorithms Bacteriological Techniques / methods* Corynebacterium / chemistry, classification, isolation & purification* Diphtheria / diagnosis*, microbiology Diphtheria Toxin / analysis* Germany Humans Laboratories Polymerase Chain Reaction Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods* |
| Chemical | |
Reg. No./Substance:
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0/Diphtheria Toxin |
| Comments/Corrections | |
Erratum In:
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Euro Surveill. 2010;15(44)pii:19702 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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