| Matrilin-3 activates the expression of osteoarthritis-associated genes in primary human chondrocytes. | |
| | |
MedLine Citation:
|
PMID: 19840795 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Here, we tested the matrilin-3-dependent induction of osteoarthritis-associated genes in primary human chondrocytes. Matrilin stimulation leads to the induction of MMP1, MMP3, MMP13, COX-2, iNOS, IL-1beta, TNFalpha, IL-6 and IL-8. Furthermore, we show the participation of ADAMTS4 and ADAMTS5 in the in vitro degradation of matrilin-3. We provide evidence for a matrilin-3-dependent feed-forward mechanism of matrix degradation, whereby proteolytically-released matrilin-3 induces pro-inflammatory cytokines as well as ADAMTS4 and -5 indirectly via IL-1beta. ADAMTS4 and ADAMTS5, in turn, cleave matrilin-3 and may release more matrilin-3 from the matrix, which could lead to further release of pro-inflammatory cytokines and proteases in cartilage. |
| | |
Authors:
|
Andreas R Klatt; Gabriele Klinger; Brigitte Paul-Klausch; Gertrud K??hn; Joerg H Renno; Raimund Wagener; Mats Paulsson; Joachim Schmidt; Gebhart Malchau; Klaus Wielckens |
Related Documents
:
|
19752225 - The role of macrophage-derived il-1 in induction and maintenance of angiogenesis. 9629285 - Cytokines and sickness behavior. 3883925 - Modulation of hepatocyte protein synthesis by endotoxin-activated kupffer cells. iii. e... 10704145 - The destructive action of il-1alpha and il-1beta in iddm is a multistage process: evide... 18022315 - Rrr-gamma-tocopherol induces human breast cancer cells to undergo apoptosis via death r... 7691945 - Il-10 inhibits the synthesis of migration inhibitory factor and migration inhibitory fa... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-10-17 |
Journal Detail:
|
Title: FEBS letters Volume: 583 ISSN: 1873-3468 ISO Abbreviation: FEBS Lett. Publication Date: 2009 Nov |
Date Detail:
|
Created Date: 2009-11-17 Completed Date: 2010-01-15 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 0155157 Medline TA: FEBS Lett Country: Netherlands |
Other Details:
|
Languages: eng Pagination: 3611-7 Citation Subset: IM |
Affiliation:
|
Institute for Clinical Chemistry, University of Cologne, Germany. andreas.klatt@uk-koeln.de |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
ADAM Proteins
/
metabolism Cells, Cultured Chondrocytes / cytology, drug effects*, metabolism Cyclooxygenase 2 / genetics Enzyme-Linked Immunosorbent Assay Extracellular Matrix Proteins / genetics, pharmacology* Gene Expression Regulation / drug effects* Humans Immunoblotting Interleukin-1beta / secretion Interleukin-6 / genetics Interleukin-8 / secretion Matrix Metalloproteinase 1 / genetics Matrix Metalloproteinase 13 / genetics Matrix Metalloproteinase 3 / genetics Nitric Oxide Synthase Type II / genetics Osteoarthritis / genetics* Procollagen N-Endopeptidase / metabolism Recombinant Proteins / pharmacology Reverse Transcriptase Polymerase Chain Reaction Tumor Necrosis Factor-alpha / secretion |
| Chemical | |
Reg. No./Substance:
|
0/Extracellular Matrix Proteins; 0/Interleukin-1beta; 0/Interleukin-6; 0/Interleukin-8; 0/Recombinant Proteins; 0/Tumor Necrosis Factor-alpha; 0/matrilin 3; EC 1.14.13.39/Nitric Oxide Synthase Type II; EC 1.14.99.1/Cyclooxygenase 2; EC 1.14.99.1/PTGS2 protein, human; EC 3.4.24.-/ADAM Proteins; EC 3.4.24.-/ADAMTS5 protein, human; EC 3.4.24.-/Matrix Metalloproteinase 13; EC 3.4.24.-/aggrecanase-1; EC 3.4.24.14/Procollagen N-Endopeptidase; EC 3.4.24.17/Matrix Metalloproteinase 3; EC 3.4.24.7/Matrix Metalloproteinase 1 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: A Locally-Induced Increase in Intracellular Ca2+ Propagates Cell-to-Cell in the Presence of Plasma M...
Next Document: Shaping tubular carriers for intracellular membrane transport.