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Matrigel Improves Functional Properties of Primary Human Salivary Gland Cells.
MedLine Citation:
PMID:  21189069     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Currently, there is no effective treatment available to patients with irreversible loss of functional salivary acini caused by Sjogren's syndrome or following radiotherapy for head and neck cancer. A tissue-engineered artificial salivary gland would help these patients. The graft cells for this device must establish tight junctions (TJs) in addition to being of fluid-secretory nature. This study analyzed a graft source from human salivary glands (huSG) cultured on Matrigel. Cells were obtained from parotid and submandibular glands, expanded in vitro, and then plated on either Matrigel-coated (2mg/ml) or -uncoated culture dish. Immunohistochemistry, TEM, quantitative RT-PCR, western blot, and transepithelial electrical resistance (TER) were employed. On Matrigel, huSG cells adopted an acinar phenotype by forming 3-D acinar-like units (within 24h of plating) as well as a monolayer of cells. On uncoated surfaces (plastic), huSG cells only formed monolayers of ductal cells. Both types of culture conditions allowed huSG cells to express TJ proteins (claudin-1, -2, -3, -4, occludin, JAM-A, and ZO-1) and adequate TER. Importantly 99% of huSG cells on Matrigel expressed α-amylase and the water channel protein Aquaporin-5, as compared to < 5% of huSG cells on plastic. TEM confirmed an acinar phenotype with many secretory granules. Matrigel increased the secretion of α-amylase 2 to 5 folds into the media, down-regulated certain salivary genes, and regulated the translation of acinar proteins. This 3-D in vitro serum-free cell culture method allows the organization and differentiation of huSG cells into salivary cells with an acinar phenotype.
Authors:
Ola M Maria; Anthony Zeitouni; Olga Gologan; Simon D Tran
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2010-12-28
Journal Detail:
Title:  Tissue engineering. Part A     Volume:  -     ISSN:  1937-335X     ISO Abbreviation:  -     Publication Date:  2010 Dec 
Date Detail:
Created Date:  2010-12-29     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101466659     Medline TA:  Tissue Eng Part A     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
McGill University, Dentistry, Montreal, Quebec, Canada; ola.maria@mail.mcgill.ca.
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