| Matrigel Improves Functional Properties of Primary Human Salivary Gland Cells. | |
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MedLine Citation:
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PMID: 21189069 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Currently, there is no effective treatment available to patients with irreversible loss of functional salivary acini caused by Sjogren's syndrome or following radiotherapy for head and neck cancer. A tissue-engineered artificial salivary gland would help these patients. The graft cells for this device must establish tight junctions (TJs) in addition to being of fluid-secretory nature. This study analyzed a graft source from human salivary glands (huSG) cultured on Matrigel. Cells were obtained from parotid and submandibular glands, expanded in vitro, and then plated on either Matrigel-coated (2mg/ml) or -uncoated culture dish. Immunohistochemistry, TEM, quantitative RT-PCR, western blot, and transepithelial electrical resistance (TER) were employed. On Matrigel, huSG cells adopted an acinar phenotype by forming 3-D acinar-like units (within 24h of plating) as well as a monolayer of cells. On uncoated surfaces (plastic), huSG cells only formed monolayers of ductal cells. Both types of culture conditions allowed huSG cells to express TJ proteins (claudin-1, -2, -3, -4, occludin, JAM-A, and ZO-1) and adequate TER. Importantly 99% of huSG cells on Matrigel expressed α-amylase and the water channel protein Aquaporin-5, as compared to < 5% of huSG cells on plastic. TEM confirmed an acinar phenotype with many secretory granules. Matrigel increased the secretion of α-amylase 2 to 5 folds into the media, down-regulated certain salivary genes, and regulated the translation of acinar proteins. This 3-D in vitro serum-free cell culture method allows the organization and differentiation of huSG cells into salivary cells with an acinar phenotype. |
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Authors:
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Ola M Maria; Anthony Zeitouni; Olga Gologan; Simon D Tran |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2010-12-28 |
Journal Detail:
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Title: Tissue engineering. Part A Volume: - ISSN: 1937-335X ISO Abbreviation: - Publication Date: 2010 Dec |
Date Detail:
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Created Date: 2010-12-29 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101466659 Medline TA: Tissue Eng Part A Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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McGill University, Dentistry, Montreal, Quebec, Canada; ola.maria@mail.mcgill.ca. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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