Document Detail


Maternally recruited DCP1A and DCP2 contribute to messenger RNA degradation during oocyte maturation and genome activation in mouse.
MedLine Citation:
PMID:  23136299     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The oocyte-to-zygote transition entails transforming a highly differentiated oocyte into totipotent blastomeres and represents one of the earliest obstacles that must be successfully hurdled for continued development. Degradation of maternal mRNAs, which likely lies at the heart of this transition, is characterized by a transition from mRNA stability to instability during oocyte maturation. Although phosphorylation of the oocyte-specific RNA-binding protein MSY2 during maturation is implicated in making maternal mRNAs more susceptible to degradation, mechanisms underlying mRNA degradation during oocyte maturation remain poorly understood. We report that DCP1A and DCP2, proteins responsible for decapping mRNA, are encoded by maternal mRNAs recruited for translation during maturation via cytoplasmic polyadenylation elements located in their 3' untranslated regions. Both DCP1A and DCP2 are phosphorylated during maturation, with CDC2A being the kinase likely responsible for both, although MAPK may be involved in DCP1A phosphorylation. Inhibiting accumulation of DCP1A and DCP2 by RNA interference or morpholinos decreases not only degradation of mRNAs during meiotic maturation but also transcription of the zygotic genome. The results indicate that maternally recruited DCP1A and DCP2 are critical players in the transition from mRNA stability to instability during meiotic maturation and that proper maternal mRNA degradation must be successful to execute the oocyte-to-zygote transition.
Authors:
Jun Ma; Matyas Flemr; Hynek Strnad; Petr Svoboda; Richard M Schultz
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2013-01-10
Journal Detail:
Title:  Biology of reproduction     Volume:  88     ISSN:  1529-7268     ISO Abbreviation:  Biol. Reprod.     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-01-11     Completed Date:  2013-06-05     Revised Date:  2014-02-20    
Medline Journal Info:
Nlm Unique ID:  0207224     Medline TA:  Biol Reprod     Country:  United States    
Other Details:
Languages:  eng     Pagination:  11     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Butadienes / pharmacology
Endoribonucleases / genetics,  metabolism*
Enzyme Inhibitors / pharmacology
Gene Expression Regulation, Developmental / physiology*
Mice
Nitriles / pharmacology
Oocytes / physiology*
Protein Kinase Inhibitors / pharmacology
Purines / pharmacology
RNA Stability / physiology*
RNA, Messenger / genetics,  metabolism*
Trans-Activators / genetics,  metabolism*
Grant Support
ID/Acronym/Agency:
HD022681/HD/NICHD NIH HHS; R01 HD022681/HD/NICHD NIH HHS
Chemical
Reg. No./Substance:
0/Butadienes; 0/Enzyme Inhibitors; 0/Nitriles; 0/Protein Kinase Inhibitors; 0/Purines; 0/RNA, Messenger; 0/Trans-Activators; 0/U 0126; 0/roscovitine; EC 3.1.-/Dcp2 protein, mouse; EC 3.1.-/Endoribonucleases; EC 3.1.-/smad4-interacting protein SMIF, mouse

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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